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Clinical and Experimental Obstetrics & Gynecology  2021, Vol. 48 Issue (2): 336-342    DOI: 10.31083/j.ceog.2021.02.2321
Original Research Previous articles | Next articles
Long non-coding RNA SNHG22 regulated by SP1 is a potential biomarker for epithelial ovarian cancer diagnosis and regulates cell glycolysis
Xiao-Yan Liu1, †, Li Deng2, †, Xiao-Ling Wu3, Xiao-Jing Tong4, *()
1Department of Teaching & Student affairs, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, 110042 Liaoning, China
2Department of Chinese Medicine, The Affiliated Hospital of Liaoning University of traditional Chinese Medicine, 116600 Liaoning, China
3Department of Gynecology, the Second People's Hospital of Baise, 533000 Guangxi, China
4Department of Gynecology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, 110042 Liaoning, China
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Abstract  
Object: Long non-coding RNAs (lncRNAs) exert critical roles in cancer progression. However, the function of SNHG22 in epithelial ovarian carcinoma (EOC) still needs to be clarified. Methods: qRT-PCR was carried out to explore SNHG22 expression. Receiver operating characteristic (ROC) curve was used to evaluate the predictive ability. Glucose uptake and lactate secretion were used to monitor cancer cell glycolysis. Luciferase reporter and ChIP assays were conducted to investigate the molecular mechanism of SNHG22. Results: SNHG22 was found notably overexpressed in tumor tissues, cells and serum samples, which was regulated by SP1 with the stimulation of SNHG22 promoter activity. And SNHG22 expression in serum was an effective biomarker by ROC analysis. Moreover, SNHG22 facilitated glycolysis in EOC cells. Conclusions: Aberrant SNHG22 expression in serum could be used as a promising biomarker for EOC and SNHG22 promotes in glycolysis.
Key words:  Long non-coding RNA      SP1      Epithelial ovarian carcinoma      Glycolysis     
Submitted:  10 October 2020      Revised:  28 November 2020      Accepted:  18 December 2020      Published:  15 April 2021     
Fund: 
2019-ZD-0588/Natural Science Foundation of Liaoning Province
201170/Shenyang Science and Technology Project
*Corresponding Author(s):  Xiao-Jing Tong     E-mail:  adnikenike@sina.com
About author:  These authors contributed equally.

Cite this article: 

Xiao-Yan Liu, Li Deng, Xiao-Ling Wu, Xiao-Jing Tong. Long non-coding RNA SNHG22 regulated by SP1 is a potential biomarker for epithelial ovarian cancer diagnosis and regulates cell glycolysis. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(2): 336-342.

URL: 

https://ceog.imrpress.com/EN/10.31083/j.ceog.2021.02.2321     OR     https://ceog.imrpress.com/EN/Y2021/V48/I2/336

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