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Clinical and Experimental Obstetrics & Gynecology  2021, Vol. 48 Issue (2): 270-277    DOI: 10.31083/j.ceog.2021.02.2314
Original Research Previous articles | Next articles
Comparison of extracellular matrix proteins expressed on stromal cells derived from human endometrium with and without spontaneous abortion
Jie Ohn Sohn1, 2, †, Hye Jin Park3, †, Se Hee Kim2, Yoon Mi Jo2, Min Ji Kim2, Hyun Jin Song2, Jung Im Yun4, 5, Jeong Mook Lim1, 6, *(), Seung Tae Lee3, 5, 7, *()
1Department of Agricultural Biotechnology, Seoul National University, 151-921 Seoul, Republic of Korea
2Fertility Medical Center, Seoul Women's Hospital, 14544 Bucheon, Republic of Korea
3Department of Animal Life Science, Kangwon National University, 24341 Chuncheon, Republic of Korea
4Institute of Animal Resources, Kangwon National University, 24341 Chuncheon, Republic of Korea
5Kustogen Inc., 24341 Chuncheon, Republic of Korea
6Research Institute of Agriculture and Life Sciences, Seoul National University, 151-921 Seoul, Republic of Korea
7Department of Applied Animal Science, Kangwon National University, 24341 Chuncheon, Republic of Korea
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Abstract  
We explored the correlation between extracellular matrix (ECM) components and spontaneous abortion by defining the types and levels of ECM proteins that are transcriptionally and translationally expressed in endometrial stromal (ES) cells. The ES cells were retrieved from the uterus of a woman who had experienced normal delivery without spontaneous abortion and one who had experienced spontaneous abortion. In the presence and absence of spontaneous abortion, the transcription of eight ECM protein-encoding genes [fibronectin, nidogen-1, tenascin C, vitronectin, elastin, collagen type 1 alpha 1 chain (COL1A1), COL3A1, and COL5A2] was observed, whereas four ECM protein-encoding genes (laminin, COL2A1, COL4A3, and COL11A1) were not transcribed. Translation of all ECM protein-encoding genes was equally observed on the surface of ES cells derived from women with normal pregnancy and spontaneous abortion; however, the proportion of ES cells expressing each ECM protein and the quantity of each ECM protein expressed on the cell surface were significantly different. These results demonstrate that altered expression of ECM proteins in ES cells constituting the endometrium may be a key factor in triggering spontaneous abortion.
Key words:  Endometrial stromal cells      Spontaneous abortion      Extracellular matrix proteins     
Submitted:  06 October 2020      Revised:  27 November 2020      Accepted:  27 November 2020      Published:  15 April 2021     
Fund: 
NRF-2017R1A2B4009777/Ministry of Science, ICR & Future Planning
*Corresponding Author(s):  limjm@snu.ac.kr (Jeong Mook Lim); stlee76@kangwon.ac.kr (Seung Tae Lee)   
About author:  These authors contributed equally.

Cite this article: 

Jie Ohn Sohn, Hye Jin Park, Se Hee Kim, Yoon Mi Jo, Min Ji Kim, Hyun Jin Song, Jung Im Yun, Jeong Mook Lim, Seung Tae Lee. Comparison of extracellular matrix proteins expressed on stromal cells derived from human endometrium with and without spontaneous abortion. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(2): 270-277.

URL: 

https://ceog.imrpress.com/EN/10.31083/j.ceog.2021.02.2314     OR     https://ceog.imrpress.com/EN/Y2021/V48/I2/270

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