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Clinical and Experimental Obstetrics & Gynecology  2019, Vol. 46 Issue (2): 231-234    DOI: 10.12891/ceog4504.2019
Original Research Previous articles | Next articles
Effect of laser-assisted three area zona thinning at the 8-cell embryo on the hatching of mouse blastocysts
J.-K. Joo1, J.-E. Jeong1, H.-G. Kim1, S.-C. Kim1, C.-W. Kim2, K.-S. Lee1, *()
1Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University School of Medicine, Busan, Korea
2Department of Obstetrics and Gynecology, Sungkyunkwan University School of Medicine Samsung Changwon Hospital, Changwon, Korea
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Abstract  

Introduction: This study was aimed to preliminarily examine the effect of laser-assisted three area zona thinning (LAZT) during the cleavage stage of embryo development and the hatching process in mice based on female age. Materials and Methods: C57BL female mice of two different age groups (6-11 and 28-31 weeks) were superovulated and then mated. The 8-cell stage embryos were treated with LAZT in three areas of zona pellucida (ZP) at 120o intervals. The control group was embryos without LAZT. Results: In old mice, LAZT significantly increased blastocyst formation and hatching compared to the control group (82% vs. 38% in number of expanding blastocyst and 66% vs. 20% in number of hatching blastocyst). Conclusion: These results show that multi-area LAZT results in a significant improvement of blastocyst formation and hatching in mice compared to controls.

Key words:  : Hatching      IVF      Laser     
Published:  10 April 2019     
*Corresponding Author(s):  K.-S. LEE     E-mail:  kuslee@pusan.ac.kr

Cite this article: 

J.-K. Joo, J.-E. Jeong, H.-G. Kim, S.-C. Kim, C.-W. Kim, K.-S. Lee. Effect of laser-assisted three area zona thinning at the 8-cell embryo on the hatching of mouse blastocysts. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(2): 231-234.

URL: 

https://ceog.imrpress.com/EN/10.12891/ceog4504.2019     OR     https://ceog.imrpress.com/EN/Y2019/V46/I2/231

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