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Clinical and Experimental Obstetrics & Gynecology  2020, Vol. 47 Issue (5): 749-754    DOI: 10.31083/j.ceog.2020.05.5362
Original Research Previous articles | Next articles
Increasd mRNA expression of TRAF-6 and MST-4 in the placenta of women with preterm premature rupture of membranes with histological chorioamnionitis
D. Lu1, *(), X. Bao1, Q. Wang1
1Department of Obstetrics and Gynecology, Clinical Medical College of Yangzhou University, Yangzhou, P.R. China
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Abstract  

Objectives: To investigate the expression of mammalian Ste20-like kinase 4 (MST-4) and tumour necrosis factor receptor-associated factor 6 (TRAF-6) in placenta from cases with preterm premature rupture of membranes (PPROM) with histological chorioamnionitis (HCA). Methods: Forty PPROM pregnant women and 40 normal controls were recruited. The foetal membranes were examined by haematoxylin-eosin staining and immunohistochemistry (IHC). MST-4 and TRAF-6 mRNA levels were measured by real-time qPCR. Results: IHC showed that the expressions of TRAF-6 and MST-4 in the combined PPROM and intrauterine infection group were significantly stronger than those in the control group and the PPROM group (p < 0.05). RT-qPCR showed that the expression of TRAF6 and MST4 mRNA in the PPROM with HCA group was significantly higher than that in the control group and the PPROM without HCA group (p < 0.05), but there was no significant difference between the latter two groups (p > 0.05). Conclusion: Increased mRNA expression of TRAF-6 and MST-4 are associated with PPROM with HCA, which may provide new detection markers for the early diagnosis of PPROM with HCA.

Key words:  Preterm premature rupture of membranes      Histological chorioamnionitis      Tumour necrosis factor receptor-associated factor 6      Mammalian Ste20-like kinase 4     
Submitted:  20 August 2019      Accepted:  09 March 2020      Published:  15 October 2020     
Fund: YZ2017075/Science and Technology Project Fund of Yangzhou;YB201972/Traditional Chinese Medicine Science and Technology Development Plan Project of Jiangsu Province
*Corresponding Author(s):  D. Lu     E-mail:  ludan1968@126.com

Cite this article: 

D. Lu, X. Bao, Q. Wang. Increasd mRNA expression of TRAF-6 and MST-4 in the placenta of women with preterm premature rupture of membranes with histological chorioamnionitis. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(5): 749-754.

URL: 

https://ceog.imrpress.com/EN/10.31083/j.ceog.2020.05.5362     OR     https://ceog.imrpress.com/EN/Y2020/V47/I5/749

Figure 1.  — Haematoxylin-eosin staining of foetal membranes (× 40) A: HCA; B: without HCA.

Table 1  — Clinical characteristics of the study population.
PPROM witd HCA (n = 34) PPROM witdout HCA (n = 6) controls (n = 40) p
Maternal age (years) 27.12 ± 3.44 25.83 ± 1.72 26.83 ± 2.48 0.61
BMI (kg/m2) 27.86 ± 2.80 27.05 ± 2.47 27.84 ± 3.84 0.64
White blood cell number (× 109) 9.64 ± 2.35 9.57 ± 2.02 9.09 ± 1.96 0.54
Neutrophil number (× 109) 7.04 ± 2.23 7.04 ± 2.23 6.55 ± 2.02 0.39
Maternal temperature (°C) 36.73 ± 0.21 36.74 ± 0.25 36.76 ± 0.22 0.88
Figure 2.  — Expression of TRAF6 and MST4 in placental tissue (× 40). A-D: expression of TRAF6 in PBS control, normal upper control, PPROM not combined with intrauterine infection group, PPROM combined with intrauterine infection group; E-H: expression of MST4 in PBS control, normal initial control, PPROM not combined with intrauterine infection group, and PPROM combined with intrauterine infection group.

Table 2  — Expression of MST-4 and TRAF-6 mRNA.
Control (n = 40) PPROM witd HCA (n = 34) p PPROM witdout HCA (n = 6) p
TRAF6 0.580 ± 0.135 1.029 ± 0.215 < 0.01 0.599 ± 0.093 0.74
MST4 0.534 ± 0.114 0.952 ± 0.182 < 0.01 0.580 ± 0.135 0.37
Figure 3.  — The expression of TRAF-6 mRNA in the PPROM with HCA group (Group A), PPROM without HCA group (Group B) and control group (Group C) was investigated by RT-PCR. The figure shows that compared with the PPROM without HCA group and the control group, the PPROM with HCA group has greater statistical significance. Group A, n = 34; Group B, n = 6; Group C, n = 40; * * * p < 0.001.

Figure 4.  — The expression of MST-4 mRNA in the PPROM with HCA group (Group A), PPROM without HCA group (Group B) and control group (Group C) was investigated by RT-PCR. The figure shows that compared with the PPROM without HCA group and the control group, the PPROM with HCA group has greater statistical significance. Group A, n = 34; Group B, n = 6; Group C, n = 40; * * * p < 0.001.

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