Please wait a minute...
Clinical and Experimental Obstetrics & Gynecology  2020, Vol. 47 Issue (3): 432-433    DOI: 10.31083/j.ceog.2020.03.5077
Case Report Previous articles | Next articles
Purposeful cryopreservation of sperm prior to intrauterine insemination to overcome the embryo implantation defect associated with sperm with a subnormal hypoosmotic swelling test
J.H. Check1, 2, *(), D.L. Check2, M.P. Dougherty3, G. DiAntonio2
1Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology & Infertility, Cooper Medical School of Rowan University, Camden, NJ, USA
2Cooper Institute for Reproductive Hormonal Disorders, P.C. Mt. Laurel, NJ, USA
3Department of Obstetrics, Gynecology And Reproductive Sciences Rutgers Robert Wood Johnson Medical School, Robert Wood Hohnson Univeristy Hospital, New Brunswick, NJ, USA
Download:  PDF(2065KB)  ( 252 ) Full text   ( 14 )
Export:  BibTeX | EndNote (RIS)      
Abstract  

Purpose: To determine if the toxic protein causing sperm to have low hypoosmotic swelling (HOS) test score and subsequent embryo implantation defects caused by this abnormality is cryolabile, and thus possibly purposeful cryopreservation followed by intrauterine insemination (IUI) could result in a pregnancy. Materials and Methods: The etiologic factor for a two-year history of infertility failing to respond to IUI and two cycles of in vitro fertilization-embryo transfer (IVF-ET) using conventional insemination was found secondary to a very low HOS test (32% with abnormal < 50%). With IVF-ET with intracytoplasmic sperm injection (ICSI), the patient conceived in her first cycle but she had a miscarriage. She failed to conceive in cycle 2 (actually her 4th IVF-ET cycle). Treatment with the protein digestive enzyme chymotrypsin corrected the HOS defect (HOS test 75-80%) on 3 different treatment specimens but she failed to conceive despite IUI with treated sperm. She had switched to IUI for financial reasons. Unfortunately the manufacturer temporarily stopped making chymotrypsin. A unique treatment was attempted for the first time - cryopreservation of the sperm prior to IUI hoping the toxic protein was cryolabile. Results: A viable pregnancy was achieved following the first IUI cycle with frozen thawed sperm. Unfortunately there was fetal demise found to be secondary to trisomy 14. Conclusion: Sperm with low HOS tests do not inhibit fertilization but instead causes embryo implantation defects hypothesized to be related to the supernumerary sperm attached to the zona pellucida. This is the first reported case of a pregnancy achieved by IUI using cryopreserved sperm with low HOS scores.

Key words:  Cryolabile      Cryopreservation      Sperm      Hypo-osmotic swelling test     
Submitted:  03 November 2018      Accepted:  17 November 2018      Published:  15 June 2020     
*Corresponding Author(s):  JEROME H. CHECK     E-mail:  laurie@ccivf.com

Cite this article: 

J.H. Check, D.L. Check, M.P. Dougherty, G. DiAntonio. Purposeful cryopreservation of sperm prior to intrauterine insemination to overcome the embryo implantation defect associated with sperm with a subnormal hypoosmotic swelling test. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(3): 432-433.

URL: 

https://ceog.imrpress.com/EN/10.31083/j.ceog.2020.03.5077     OR     https://ceog.imrpress.com/EN/Y2020/V47/I3/432

[1] Check J.H., Epstein R., Nowroozi K., Shanis B.S., Wu C.H., Bollendorf A.: “The hypo-osmotic swelling test as a useful adjunct to the semen analysis to predict fertility potential”. Fertil. Steril., 1989, 52, 159.
doi: 10.1016/s0015-0282(16)60808-7 pmid: 2744184
[2] Kiefer D., Check J.H., Katsoff D.: “The value of motile density, strict morphology, and the hypoosmotic swelling test in in vitro fertilization-embryo transfer”. Arch. Androl., 1996, 37, 57.
doi: 10.3109/01485019608988502 pmid: 8827348
[3] Check J.H., Stumpo L., Lurie D., Benfer K., Callan C.: “A comparative prospective study using matched samples to determine the influence of subnormal hypo-osmotic test scores of spermatozoa on subsequent fertilization and pregnancy rates following in vitro fertilization”. Hum. Reprod., 1995, 10, 1197.
doi: 10.1093/oxfordjournals.humrep.a136118 pmid: 7657765
[4] Katsoff D., Check M.L., Check J.H.: “Evidence that sperm with low hypoosmotic swelling scores cause embryo implantation defects”. Arch. Androl., 2000, 44, 227.
doi: 10.1080/014850100262218 pmid: 10864371
[5] Check J.H., Katsoff D., Check M.L.: “Some semen abnormalities may cause infertility by impairing implantation rather than fertilization”. Med. Hypoth., 2001, 56, 653.
[6] Check J.H., Katsoff D., Check M.L., Choe J.K., Swenson K.: “In vitro fertilization with intracytoplasmic sperm injection is an effective therapy for male factor related to subnormal hypo-osmotic swelling test scores”. J. Androl., 2001, 22, 261.
pmid: 11229800
[7] Check J.H., Katsoff B., Yuan W., Summers-Chase D., Hourani W., Horwath D.: “Intracytoplasmic sperm injection completely negates the implantation problem associated with conventional fertilization with sperm with low hypo-osmotic swelling test scores as evidenced by evaluating donor-recipient pairs”. Clin. Exp. Obstet. Gynecol., 2012, 39, 21.
pmid: 22675948
[8] Katsoff D., Check J.H.: “Two methods of achieving pregnancies despite subnormal hypo-osmotic swelling test scores”. Fertil. Steril., 1997, 68, 549.
doi: 10.1016/s0015-0282(97)00234-3 pmid: 9314933
[9] Citrino G., Check J.H., Diantonio A., Bollendorf A., Katsoff D.: “Pretreatment of sperm with low hypo-osmotic swelling tests with chymotrypsin prior to intrauterine insemination (IUI) and avoidance of unprotectected intercourse results in pregnancy rates comparable to IUI for other male factor problems”. Clin. Exp. Obstet. Gynecol., 2010, 37, 187.
pmid: 21077521
[10] Check M.L., Katsoff D., Check J.H., Summers-Chase D.: “Effect of treating sperm with low hypo-osmotic swelling test scores with chymotrypsin on pregnancy rates after conventional in vitro fertilization-embryo transfer”. Fertil. Steril., 2004, 82, 741.
doi: 10.1016/j.fertnstert.2004.05.066
[11] Bollendorf A., Check D., Check J.H., Hourani W., McMonagle K.: “A comparison of the efficacy of treating sperm with low hypo-osmotic swelling test scores with chymotrypsin followed by intrauterine insemination vs. in vitro fertilization with intracytoplasmic sperm injection”. Clin. Exp. Obstet. Gynecol., 2011, 38, 24.
pmid: 21485719
[12] Check J.H., Baker A., Benfer K., Lurie D., Katsoff D.: “Transfer of cryopreserved embryos improved pregnancy rates in patients with damage to the functional integrity of the sperm membrane as measured by the hypo-osmotic swelling test”. Fertil. Steril., 1996, 65, 1241.
doi: 10.1016/s0015-0282(16)58348-4 pmid: 8641507
[1] Sema Karakaş, Cihan Kaya, Sebastian Daniel Schäfer, Mislav Mikuš, Milan Terzic, Bekhzod Abdullaev, Alessandra Andrisani. Fertility-sparing in cancer patients[J]. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(4): 787-794.
[2] Mitra Rafiee, Nasrin Sereshki, Razieh Alipour, Vahid Ahmadi Panah, David Wilkinson. Lack of anti-paternal cytotoxic antibody production in the presence of spermatozoa in recurrent spontaneous abortion couples in contrast to fertile couples: a pilot study[J]. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(3): 578-582.
[3] I. Thanaboonyawat, P. Chera-aree, S. Petyim, R. Choavaratana, P. Laokirkkiat. The effect of three-month topical testosterone gel application on semen quality in men with oligozoospermia and low serum testosterone levels[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(6): 875-881.
[4] K. Okuno, K. Kitaya, N. Kim, K. Yamaguchi, H. Matsubayashi, T. Ishikawa. Case report of successful live birth in an infertile couple with male idiopathic hypogonadotropic hypogonadism/azoospermia and atypical polypoid adenomyoma of the uterus[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(6): 972-973.
[5] T. Toporcer, T. Grendel, I. Uhrinová, S. F. Iannaccone, A. Grendelová, F. Sabol, J. Belák, S. Toporcerová. Effect of low molecular weight heparin on autotransplantation efficacy of ovarian tissues in rats[J]. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(4): 542-548.
[6] Yin Shao, Liguo Ma, Meiyi Chen, Yuxia Guo, Hongtao Xiao. The effect of VEGF and Ang-1 on cryopreserved human ovarian grafts in severe combined immunodeficient mice[J]. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(3): 377-382.
[7] H. Ueda, G. Minase, T. Miyamoto, M. Iijima, Y. Saijo, M. Nakashima, N. Matsumoto, M. Namiki, K. Sengoku. Single-nucleotide polymorphisms in ETV5: a risk factor for Sertoli cell-only syndrome in Japanese men?[J]. Clinical and Experimental Obstetrics & Gynecology, 2018, 45(2): 187-189.
[8] T. Miyamoto, K. Abiko, A. Itabashi, G. Minase, H. Ueda, K. Sengoku. MD-TESE-ICSI using fresh sperm resulted in a lower rate of miscarriage compared with frozen-thawed sperm[J]. Clinical and Experimental Obstetrics & Gynecology, 2018, 45(2): 229-230.
[9] M. Karacan, A. Usta, A. Arvas, E. Erdem, M. Ulug, Z. Cebi, T. Camlibel. The effect of coasting on the outcome of intracytoplasmic sperm injection: comparable analysis of GnRH agonist and antagonist cycles[J]. Clinical and Experimental Obstetrics & Gynecology, 2018, 45(1): 48-52.
[10] H. Chen, X. Gao, L.L. Shi, X.B. Gao. Cytogenetic analysis of 10,286 cases with male infertility[J]. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(3): 392-394.
[11] J.H. Check, A. Bollendorf. The correlation of the degree of abnormal sperm morphology using strict criteria and pregnancy rates following intrauterine insemination (IUI)[J]. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(2): 183-184.
[12] C. Sofoudis, I. Zervomanolakis, I. Galani, V. Grigoriou, D. Botsis, N. Vlahos. Assessment of follicle viability using fluorescence microscopy before and after ovarian thawing[J]. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(2): 305-309.
[13] P. Korrovits, E. Lapp, R. Mändar. Couple-related factors of ART outcome[J]. Clinical and Experimental Obstetrics & Gynecology, 2016, 43(5): 747-750.
[14] J. H. Check, J. Aly. Sperm with an abnormal hypo-osmotic swelling test – normal fertilization, normal embryo development, but implantation failure[J]. Clinical and Experimental Obstetrics & Gynecology, 2016, 43(3): 319-327.
[15] A. Bollendorf, J. H. Check. Correlation of ImmunoBead® and ImmunoSphere Immunoglobulin G (IGG) tests on detecting antisperm antibody (ASA) on sperm[J]. Clinical and Experimental Obstetrics & Gynecology, 2016, 43(2): 175-177.
No Suggested Reading articles found!