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Clinical and Experimental Obstetrics & Gynecology  2019, Vol. 46 Issue (3): 377-382    DOI: 10.12891/ceog4617.2019
Original Research Previous articles | Next articles
The effect of VEGF and Ang-1 on cryopreserved human ovarian grafts in severe combined immunodeficient mice
Yin Shao1, Liguo Ma1, Meiyi Chen1, *(), Yuxia Guo1, Hongtao Xiao1
1Department of Gynecology, Shenzhen People's Hospital, Second Affiliated Hospital of Jinan University, Shenzhen, China
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Abstract  

This study investigates the independent and combined effect of vascular epithelial growth factor (VEGF) and angiopoietin 1 (Ang-1) on the follicle survival and vasculogenesis of cryopreserved human ovarian grafts in severe combined immunodeficient (SCID) mice. Cryopreserved human ovarian tissue was transplanted into the thigh of SCID mice with treatment of VEGF, Ang-1, or combination at the site of transplantation. Number of follicles, morphology change, follicle apoptosis, micro-vessel density (MVD), and expression of follicle development related genes were assessed in each transplanted ovarian group of SCID mice. VEGF and Ang-1 treatments increased functional follicles and MVD value, reduced the level of follicle apoptosis, and modulated expression of ovarian development related genes and FSH level. Synergistic effect of VEGF and Ang-1 on follicle survival was significantly stronger than treatment alone. In conclusion, treatment of VEGF and Ang-1 significantly improved the survival of follicles and vasculogenesis of transplanted human ovarian tissue in SCID mice.

Key words:  VEGF      Ang-1      Cryopreservation      Ovarian      Hetero-transplantation      Survival of follicles      Vasculogenesis     
Published:  10 June 2019     
*Corresponding Author(s):  MEIYI CHEN     E-mail:  doctorsy0755@163.com

Cite this article: 

Yin Shao, Liguo Ma, Meiyi Chen, Yuxia Guo, Hongtao Xiao. The effect of VEGF and Ang-1 on cryopreserved human ovarian grafts in severe combined immunodeficient mice. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(3): 377-382.

URL: 

https://ceog.imrpress.com/EN/10.12891/ceog4617.2019     OR     https://ceog.imrpress.com/EN/Y2019/V46/I3/377

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[2] S.H. Park, J. Lim, K.T. Lim, K.H. Choi, S.M. Yoon, H.J. Chung. Ovarian teratoma with anti-N-methyl-D-aspartate receptor encephalitis, a type of limbic encephalitis: a review of the literature and a case report in Korea[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(5): 774-780.
[3] Y.A. Kim. Mature cystic teratoma of the uterus presenting as an endometrial polyp in a patient with a history of ovarian mature cystic teratoma with gliomatosis peritonei and ovarian endometriosis: a case report[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(5): 800-804.
[4] B.G. Kadioglu, B. Gundogdu, N. Kurt, A. Ozbek Bilgin, H. Suleyman, Z. Suleyman. The effect of rhodiola rosea root extract on stress-induced ovarian damage, infertility and reproductive disorders in female rats[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(4): 530-536.
[5] Y.J. Shin, S. Lee, J.E. Lee, S. Won, M.J. Kim. Clinical presentation, hormonal profiles in nulliparous Korean women with polycystic ovarian morphology[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(3): 359-364.
[6] R. Robeva, S. Andonova, M. Glushkova, T. Todorov, A. Elenkova, A. Savov, S. Zacharieva, A. Todorova. A rare BMP15 genetic variant in a patient with premature ovarian insufficiency and two spontaneous pregnancies[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(3): 409-411.
[7] T.N. Yu, Y.L. Liu, Y.X. Lee, C.R. Tzeng, C.H. Chen. Vibration-related ovarian torsion in a patient undergoing in vitro fertilization: A case report[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(3): 415-418.
[8] X. Yin, Z.Y. Wang, Y. Yao. Acute kidney injury secondary to tubo-ovarian abscess: A case report[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(3): 421-423.
[9] J.H. Check, D.L. Check, M.P. Dougherty, G. DiAntonio. Purposeful cryopreservation of sperm prior to intrauterine insemination to overcome the embryo implantation defect associated with sperm with a subnormal hypoosmotic swelling test[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(3): 432-433.
[10] H. Saleh, F. Moiety, A.F. Agameya, Y. Elkassar, R.M. El Sharakwy, D. Zeidan, H. Elmeligy. Comparison between antral follicle count and anti-Müllerian hormonal level in the prediction of ovarian response and pregnancy outcome in intracytoplasmic sperm injection patients: implications in personalizing ovarian stimulation[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(2): 166-173.
[11] S. Dikić, S. Dragojević, L. Nejković, J. Štulić, A. Juri šić, D. Radojičić, A. Diki ć, Dj Radak. Management of the postpartum ovarian and partial cava inferior vein thrombosis[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(2): 299-302.
[12] S.Y. Jung, H.Y. Cho, S.H. Lee, S. Lim, K.B. Lee. Pregnancy outcomes of a giant primary ovarian leiomyoma in the first trimester[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(1): 135-138.
[13] F. Sorrentino, F. Boncristiano, F. Greco, A. D’Aloia, P. Di Gioia, L. Nappi. Primary borderline paraovarian serous tumor in pregnancy: case report and review of the literature[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(1): 139-141.
[14] N. Smorgick, O. Nir, M. Pekar-Zlotin, S. Maymon, Y. Melcer, R. Maymon. Adnexal torsion: is there a familial tendency?[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(1): 62-64.
[15] Hue HJ, Kim SK, Choi JY, Suh DH, Kim KD, No JH, Lee JR, Jee BC, Kim YB, Jeon HW. Previous ovarian surgery increases the risk of tubal factor infertility[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(1): 84-88.
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