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Clinical and Experimental Obstetrics & Gynecology  2019, Vol. 46 Issue (1): 32-35    DOI: 10.12891/ceog4369.2019
Original Research Previous articles | Next articles
Comparison of BD Affirm VPIII with Gram and liquid-based cytology for diagnosis of bacterial vaginoses, candidiasis and Trichomonas
J. Eleutério Junior1, *(), R.M. Nunes Eleutério2, A.B. Gordiano Vasconcelos Valente3, F. Soares Queiroz3, A.K. Silveira Gonçalves4, P.C. Giraldo5
1 Department of Maternal and Child Health, Federal University of Ceará, Fortaleza, CE
2 Eleutério Laboratory, Federal University of Ceará (UFC), Fortaleza, CE
3 Medical School, Federal University of Ceará (UFC), Fortaleza, CE
4 Gynecology and Obstetrics Department, Federal University of Rio Grande do Norte, Natal, Brazil
5 Gynecology and Obstetrics Department, State University of Campinas, Campinas, Brazil
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Abstract  
Background: Vaginitis require complementary methods for adequate diagnosis. New technologies have been developed and need to be compared with those already established. Aims: The objective of this study was to compare the results of BD Affirm VPIII with Gram smear and liquid-based cytology in diagnosis of bacterial vaginosis (BV), Candida sp, and Trichomonas vaginalis. Materials and Methods: A cross-sectional study was performed in 90 women from March to November 2016 in a gynecology clinic in a Brazilian city, Fortaleza. The Affirm test, Gram, and Surepath were performed in vaginal samples. Categorical data was evaluated for sensibility, specificity, and predictive values. To quantify the agreement, the Kappa index was utilized. Results: Comparing Affirm test with Gram stain for BV and Surepath, the sensitivity was 83.3% and 27.8%, and the specificity was 62.2% and 95.5%, respectively. The Kappa index was 0.204 to Gram and 0.247 to Surepath. For Candida sp comparing with Gram and Surepath, the sensitivity was 61.1% and 56.3%, and the specificity was 93.3% and 100%, respectively. The Kappa index was 0.585 to Gram and 0.677 to Surepahth. Conclusion: The Affirm test had a fair concordance with Gram and Surepath in the diagnosis of BV, and substantial agreement in the diagnosis of Candida.
Key words:  Molecular diagnostic techniques      Cytodiagnosis      Vaginitis      Vaginosis      Vulvovaginal candidiases      Trichomonas infection     
Published:  10 February 2019     
*Corresponding Author(s):  J. ELEUTéRIO JUNIOR     E-mail:  prof.eleuterio@gmail.com

Cite this article: 

J. Eleutério Junior, R.M. Nunes Eleutério, A.B. Gordiano Vasconcelos Valente, F. Soares Queiroz, A.K. Silveira Gonçalves, P.C. Giraldo. Comparison of BD Affirm VPIII with Gram and liquid-based cytology for diagnosis of bacterial vaginoses, candidiasis and Trichomonas. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(1): 32-35.

URL: 

https://ceog.imrpress.com/EN/10.12891/ceog4369.2019     OR     https://ceog.imrpress.com/EN/Y2019/V46/I1/32

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