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Clinical and Experimental Obstetrics & Gynecology  2018, Vol. 45 Issue (6): 875-879    DOI: 10.12891/ceog4351.2018
Original Research Previous articles | Next articles
Decreased cell-free but not exosomal miR-518b in maternal plasma is caused by amniocentesis
K. Miura1, *(, A. Higashijima1, Y. Hasegawa1, S. Miura1, KI. Yoshiura2, H. Masuzaki1
1 Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
2 Department of Human Genetics, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
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Abstract  
Aim: To obtain knowledge of the relationship between cell-free and exosomal pregnancy-associated microRNAs in maternal plasma, the authors investigated their circulating levels before and after amniocentesis. Materials and Methods: In 16 maternal plasma samples collected before and after amniocentesis, circulating levels of cell-free and exosomal pregnancy-associated microRNAs (miR-515-3p, -517a, -517c, -518b, and 323-3p) were measured by real-time quantitative RT-PCR. Changes in the plasma concentration and the association between circulating levels of cell-free and exosomal microRNAs were analyzed. Statistical significance was defined as p < 0.05. Results: The plasma concentration of cell-free miR-518b was significantly decreased after amniocentesis (Wilcoxon signed rank test, p = 0.001), but no significant difference was observed in any other cell-free or exosomal microRNA (p > 0.05). There was no association between the circulating levels of cell-free and exosomal microRNAs (miR-515-3p, -517a, -517c, -518b) in chromosome 19 microRNA cluster region. However, a significant association was detected between plasma cell-free and exosomal miR-323-3p levels in chromosome 14 microRNA cluster region (r and P values before amniocentesis; 0.617 and 0.011, those after amniocentesis; 0.899 and < 0.001). Conclusions: Amniocentesis caused a decreased cell-free but not exosomal miR-518b levels in maternal plasma. Correlations between plasma concentrations of cell-free and exosomal pregnancy-associated microRNAs were different for each microRNA.
Key words:  MicroRNA      Amniocentesis      Cell-free      Exosome      Maternal plasma     
Published:  10 December 2018     
*Corresponding Author(s):  K. MIURA     E-mail:  kiyonori@nagasaki-u.ac.jp

Cite this article: 

K. Miura, A. Higashijima, Y. Hasegawa, S. Miura, KI. Yoshiura, H. Masuzaki. Decreased cell-free but not exosomal miR-518b in maternal plasma is caused by amniocentesis. Clinical and Experimental Obstetrics & Gynecology, 2018, 45(6): 875-879.

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https://ceog.imrpress.com/EN/10.12891/ceog4351.2018     OR     https://ceog.imrpress.com/EN/Y2018/V45/I6/875

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