Please wait a minute...
Clinical and Experimental Obstetrics & Gynecology  2018, Vol. 45 Issue (6): 817-822    DOI: 10.12891/ceog3966.2018
Original Research Previous articles | Next articles
miRNA profiling reveals the upregulation of osteogenesis-associated miRNAs in ovariectomy osteoporosis mice
He Hu1, *(), Guowei Zhang2, Geng Tian1, Guang Lv1, Yongli Jin3
1 Medical College, Inner Mongolia University for the Nationalities, Tongliao City, Inner Mongolia, China
2 College of Nursing, Inner Mongolia University for the Nationalities, Tongliao City, Inner Mongolia, China
3 The Affiliated Hospital to Inner Mongolia University for the Nationalities, Tongliao City, Inner Mongolia, China
Download:  PDF
Export:  BibTeX | EndNote (RIS)      
Abstract  
Backgrounds: Osteoporosis is a common aging-related degenerative bone disease, particularly in postmenopausal women, that have a reduced estrogen level due to aging. Various factors have been recognized to promote osteoporosis development, such as microRNAs (miRNAs), which regulate the balance between osteogenesis and osteoclasis. Aim: This study was to profile miRNAs in the ovariectomy osteoporosis mice with miRNA profiling array, and to associate the deregulated miRNAs with osteogenesis. Materials and Methods: The authors firstly profiled, with miRNAs microarray analysis, the serum miRNAs in a mouse model with postmenopausal osteoporosis, post-ovary excision. Then they validated the dysregulated miRNAs via quantitative real-time polymerase chain reaction (qRT-PCR) method. In addition, these deregulated miRNAs were subjected to a pathway analysis. Results: It was indicated by the present results that in the ovariectomized mice, ten miRNAs were upregulated, such as miR-27a-5p, miR-26a-5p, miR-106a-5p, and miR- 133a-5p and the upregulation of miR-26a-5p, miR-133a-1-5, miR-141-5p, miR-200a-5p, and miR-205-5p were validated by the followed qRT-PCR method. In addition, the pathway analysis demonstrated that these miRNAs might inhibit the bone formation via regulating osteogenesis. Conclusions: In conclusion, the authors found the upregulation of miRNAs, which downregulates osteogenesis in a mice model of postmenopausal osteoporosis. The present findings suggest the potential inhibition by miRNAs in osteogenesis in postmenopausal osteoporosis.
Key words:  miRNA profiling      Postmenopausal osteoporosis      Osteogenesis      Mice model     
Published:  10 December 2018     
*Corresponding Author(s):  HE HU     E-mail:  huhe8080@sohu.com

Cite this article: 

He Hu, Guowei Zhang, Geng Tian, Guang Lv, Yongli Jin. miRNA profiling reveals the upregulation of osteogenesis-associated miRNAs in ovariectomy osteoporosis mice. Clinical and Experimental Obstetrics & Gynecology, 2018, 45(6): 817-822.

URL: 

https://ceog.imrpress.com/EN/10.12891/ceog3966.2018     OR     https://ceog.imrpress.com/EN/Y2018/V45/I6/817

[1] Ö. Murat, Ç. Önder. The effects of exogenous melatonin administration on bone in ovariectomized and pinealectomized rats[J]. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(6): 933-937.
[2] Y. Ikeda, A. Komatsu, H. Gomibuchi. A case of postpartum multiple vertebral fractures in a patient with osteogeneis imperfecta[J]. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(5): 839-841.
No Suggested Reading articles found!