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Clinical and Experimental Obstetrics & Gynecology  2021, Vol. 48 Issue (3): 661-664    DOI: 10.31083/j.ceog.2021.03.2485
Original Research Previous articles | Next articles
The performance of GeneXpert® PCR assay in detecting group B streptococcus colonization at labor
Mohammed Rohi Khalil1, *(), Jens Kjølseth Møller2, Stine Yde Nielsen2
1Department of Gynecology and Obstetrics, Kolding Hospital, University Hospital of Southern Denmark, 6000 Kolding, Denmark
2Department of Clinical Microbiology, Vejle Hospital, University Hospital of Southern Denmark, 7100 Vejle, Denmark
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Abstract  
Background: The aim of this study was to assess the performance of GeneXpert® group B streptococcus (GBS) PCR assays in a Danish setting, using rectovaginal GBS culture at labor as the gold standard. Methods: Three hundred and sixty-six (366) women with one or more of the following risk factors for GBS carriage—GBS during current pregnancy, prior infant with EOGBS, temperature >38.0 ℃ during labor, preterm labor <37 weeks of gestation, rupture of membranes ≥18 hours—were included in the study. Intrapartum rectovaginal swab samples were tested at the bedside by GeneXpert® GBS PCR assay, and cultured on agar plates (Granada) with and without prior use of growth-selective enrichment broth. Results: The GeneXpert® GBS PCR assay showed a sensitivity of 91.7%, a specificity of 97.2%, a PPV of 92.6 %, and a NPV of 96.8%. The turnaround time of the assay was 50 minutes. Conclusions: The GeneXpert® has a high performance, indicating that the assay can be used in a clinical setting.
Key words:  Group B streptococcus      Early-onset neonatal infection      Polymerase chain reaction      Rapid intrapartum assay      GeneXpert®     
Submitted:  21 January 2021      Revised:  12 February 2021      Accepted:  12 March 2021      Published:  15 June 2021     
*Corresponding Author(s):  Mohammed Rohi Khalil     E-mail:  mohammed.khalil@rsyd.dk

Cite this article: 

Mohammed Rohi Khalil, Jens Kjølseth Møller, Stine Yde Nielsen. The performance of GeneXpert® PCR assay in detecting group B streptococcus colonization at labor. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(3): 661-664.

URL: 

https://ceog.imrpress.com/EN/10.31083/j.ceog.2021.03.2485     OR     https://ceog.imrpress.com/EN/Y2021/V48/I3/661

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