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Clinical and Experimental Obstetrics & Gynecology  2019, Vol. 46 Issue (5): 797-799    DOI: 10.12891/ceog4992.2019
Original Research Previous articles | Next articles
Inhibin B level changes during the follicular phase in rats with unilateral ovarian torsion
S.B. Cohen1, *(), R. Schonmann1, J. Boaziz1, D.S. Seidman1, R. Orvieto1, 2, †, R. Mashiach1, †
1Departments of Obstetrics and Gynecology, Sheba Medical Center, Tel-Hashomer, Affiliated with the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
2The Tarnesby-Tarnowski Chair for Family Planning and Fertility Regulation, at the Sackler Faculty of Medicine, Tel-Aviv University, Israel
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Abstract  

Objective: To investigate the association between duration of ovarian torsion and levels of serum inhibin B in an animal model. Materials and Methods: An animal model prospective study was conducted in a tertiary referral hospital and chemical pathology institute. Nineteen female Lewis rats were divided to four groups. In the first group, the control group, laparotomies alone were performed. In groups 2-4 laparotomies and right ovarian torsion for 720 degrees with fixation of the adnexa to the parietal peritoneum were performed. Blood samples were taken from the rat groups at 12, 24, and 36 hours after laparotomy. Inhibin B levels were measured by ELISA. Results: Serum mean ± SD inhibin B level in the control rats during the beginning of the metesterus, 12, 24, and 36 hours later were 108.4 ± 43.6, 129.5 ± 49.3, 99.07 ± 60.50, and 91.58 ± 27.74 pg/ml, respectively. Inhibin B levels in the control group increased 12 hours after the beginning of the metesterus, whereas in the study group, a decline in inhibin B levels was found at the same time. This difference was significant (p = 0.05). Inhibin B levels at 24 and 36 hours from the beginning of the metestrus did not show statistical difference. Conclusions: Unilateral ovarian torsion in rats changed the pattern of inhibin B secretion at the beginning of the metesterus and was associated with a significant decrease in inhibin B serum levels. This observation, following acute ovarian vascular occlusion, is probably due the effects of ischemia on hormonal regulation during the early follicular phase.

Key words:  Ovarian torsion      Inhibin B      Follicular changes      Animal model      Rats     
Published:  10 October 2019     
*Corresponding Author(s):  S.B. COHEN     E-mail:  shlomo.cohen@sheba.health.gov.il
About author:  Contributed equally.

Cite this article: 

S.B. Cohen, R. Schonmann, J. Boaziz, D.S. Seidman, R. Orvieto, R. Mashiach. Inhibin B level changes during the follicular phase in rats with unilateral ovarian torsion. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(5): 797-799.

URL: 

https://ceog.imrpress.com/EN/10.12891/ceog4992.2019     OR     https://ceog.imrpress.com/EN/Y2019/V46/I5/797

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