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Clinical and Experimental Obstetrics & Gynecology  2019, Vol. 46 Issue (4): 542-548    DOI: 10.12891/ceog4544.2019
Original Research Previous articles | Next articles
Effect of low molecular weight heparin on autotransplantation efficacy of ovarian tissues in rats
T. Toporcer1, T. Grendel2, I. Uhrinová3, S. F. Iannaccone4, A. Grendelová5, F. Sabol1, J. Belák6, S. Toporcerová7, *()
1Department of Heart Surgery, Medical faculty, Pavol Jozef Šafarik University and Eastern Slovak Institute for Cardiovascular Diseases Ltd., KoŠice, Slovakia
2Department of Anaesthesiology and Intensive Medicine, Medical faculty, Pavol Jozef Šafarik University and Eastern Slovak Institute for Cardiovascular Diseases Ltd., KoŠice, Slovakia
3Gyncare Centre of Assisted Reproduction, KoŠice, Slovakia
4Department of Forensic Medicine, Medical Faculty, Pavol Jozef Šafarik University, KoŠice, Slovakia
5Department of Gynaecology and Obstetrics, Medical Faculty, Pavol Jozef Šafarik University and 1st private Hospital KoŠice-Šaca Ltd., KoŠice, Slovakia
6Second Department of Surgery, Medical Faculty, Pavol Jozef Šafarik University and Louis Pasteur University Hospital, KoŠice, Slovakia
7Department of Gynaecology and Obstetrics, Medical Faculty, Pavol Jozef Šafarik University and Louis Pasteur University Hospital, KoŠice and Gyncare - Centre of Assisted Reproduction, KoŠice, Slovakia
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Abstract  

Background: One of the most important problems of ovarian transplantation is the significant reduction in the number of primordial follicles during the short period after transplantation due to post-transplantation ischemia. The aim of the work was to evaluate the effect of enoxaparin application on follicular survival after cryopreserved and fresh ovary transplantation in rats. Materials and Methods: The study used female Sprague Dawley rats (n=56). The animals were ovariectomized and the healing of fresh and cryopreserved ovarian tissue was evaluated. Estrogen blood level, percentage of ovarian live tissue, and angiogenesis were evaluated 16 days after transplantation of fresh ovary and 7, 14, and 28 days after transplantation of cryopreserved ovarian tissue. The animals in the experimental groups were treated after transplantation by enoxaparin subcutaneously in doses of 200 IU/kg per day. Results: A statistically significant higher (p < 0.05) percentage of live tissue was recorded in the treated goup (14.5±6.5 vs. 20.6±5.7% in control vs. treated groups) 7 days after transplantation of cryopreserved ovarian tissue, and a significantly more intensive (p<0.005) neoangiogenesis was recorded in the treated group (12.7±1.9 capillaries in 250 μm2) in comparison with the control group (7.4±2.1 capillaries in 250 μm2) 28 days after transplantation of cryopreserved ovarian tissue. Conclusions: Administration of enoxaparin after transplantation of cryopreserved ovarian tissue may temporarily improve tissue survival. The use of enoxaparin does not adversely affect neoangiogenesis around the transplanted ovary. The positive effect of low molecular weight heparin application on neoangiogenesis was demonstrated four weeks after transplantation.

Key words:  Low molecular weight heparin      Ovary autotransplantation      Cryopreservation      Angiogenesis     
Published:  10 August 2019     
*Corresponding Author(s):  S. TOPORCEROVÁ     E-mail:  s.toporcerova@gmail.com

Cite this article: 

T. Toporcer, T. Grendel, I. Uhrinová, S. F. Iannaccone, A. Grendelová, F. Sabol, J. Belák, S. Toporcerová. Effect of low molecular weight heparin on autotransplantation efficacy of ovarian tissues in rats. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(4): 542-548.

URL: 

https://ceog.imrpress.com/EN/10.12891/ceog4544.2019     OR     https://ceog.imrpress.com/EN/Y2019/V46/I4/542

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