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Clinical and Experimental Obstetrics & Gynecology  2019, Vol. 46 Issue (4): 526-530    DOI: 10.12891/ceog4483.2019
Original Research Previous articles | Next articles
Age specific reference intervals of serum anti-Müllerian hormone concentration of 1,253 women with healthy females in infertility center
L.-S. Wang1, X.-L. Zhao1, X.-A. Cai1, G.-F. Fu1, *()
1Department of Clinical Laboratory, The 105th Hospital of People Liberation Army, Hefei, Anhui Province, China
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Objective: To investigate the age specific reference intervals of serum anti-MÁllerian hormone (AMH) concentration of healthy women in infertility center. Materials and Methods: After exclusion of 642 participator who did not meet the inclusion criteria, a total of 1,253 healthy women aged 17 to 49 years were enrolled in the study between January and August 2016. This study population was divided to five age groups, The samples of this study collected from each participants and aliquoted and stored at -80℃ refrigeratory until assayed, The authors used AMH enzyme-linked immunosorbent assay (ELISA) kit for the assessment of AMH levels. Results: The serum AMH levels varied inversely correlation with patient age. The AMH concentration can be roughly calculated by formula (AMH =-0.327*age+15.98). This downward trend was significant among these women whom 40 years age. The coefficient of variation was larger even in the same age groups, The 2.5-97.5th percentile of AMH level of each age group was 2.30-16.7 ng/mL, 0.96-16.4ng/mL, 0.44-15.1ng/ mL, 0.14-12.5ng/mL, and 0.08-6.56ng/mL, respectively. Conclusions: This study determined reference values of serum AMH in China women with regular menstruation. These values can be applied to clinical evaluation and treatment of infertile women. It suggested that AMH is an individual medical index that women should establish their own baseline date for longitudinal axis comparison.

Key words:  Anti-Müllerian hormone      Reference intervals      Ovarian reserve     
Published:  10 August 2019     
*Corresponding Author(s):  G.-F. FU     E-mail:

Cite this article: 

L.-S. Wang, X.-L. Zhao, X.-A. Cai, G.-F. Fu. Age specific reference intervals of serum anti-Müllerian hormone concentration of 1,253 women with healthy females in infertility center. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(4): 526-530.

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[1] J.H. Check, E. Chang, R. Cohen. A case report supporting the concept that a role for the anti-Müllerian hormone (AMH) in normal folliculogenesis is to diminish the biological activity of follicle stimulating hormone (FSH)[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(4): 620-621.
[2] H. Saleh, F. Moiety, A.F. Agameya, Y. Elkassar, R.M. El Sharakwy, D. Zeidan, H. Elmeligy. Comparison between antral follicle count and anti-Müllerian hormonal level in the prediction of ovarian response and pregnancy outcome in intracytoplasmic sperm injection patients: implications in personalizing ovarian stimulation[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(2): 166-173.
[3] Hue HJ, Kim SK, Choi JY, Suh DH, Kim KD, No JH, Lee JR, Jee BC, Kim YB, Jeon HW. Previous ovarian surgery increases the risk of tubal factor infertility[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(1): 84-88.
[4] N. Eamudomkarn, L. Salang, K. Seejorn, P. Kleebkaow. Comparison of impact on ovarian reserve between laparoscopic and laparotomy ovarian cystectomy[J]. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(5): 779-783.
[5] R. Nishiyama, K. Kitaya, Y. Takaya, K. Yamaguchi, N. Kim, H. Matsubayashi, T. Ishikawa. Successful ongoing pregnancy following cryopreserved-thawed blastocyst transfer in an infertile Kallmann syndrome woman with balanced reciprocal translocation: a case report[J]. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(2): 331-332.
[6] A.H. Kaya, E. Tekgunduz, S. Akpınar, G. Bozdağ, T.N. Yiğenoğlu, A. Merdin, S. Namdaroglu, O. Kayıkcı, F. Altuntaş. Assessment of ovarian reserve with anti-Mullerian hormone in women following allogeneic hematopoietic cell transplantation[J]. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(1): 127-130.
[7] J. Micic, L. Surlan, J. Dotlic, N. Milic, S. Vidakovic, N. Radunovic. Ovarian response predictive model in different controlled ovarian stimulation protocols for IVF/ICSI treatment[J]. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(6): 923-928.
[8] H.U. Yuvacı, S. Uysal, H. Haltaş, B. Sırav, C.I. Duvan, N. Turhan, N. Seyhan. The effect of non-ionizing radiation on the ovarian reserves of female rats[J]. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(4): 605-610.
[9] A. Eser, D. Hizli, M. Namuslu, H. Haltas, N. Kosus, A. Kosus, H. Kafali. Protective effect of curcumin on ovarian reserve in a rat ischemia model: an experimental study[J]. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(3): 453-457.
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