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Clinical and Experimental Obstetrics & Gynecology  2019, Vol. 46 Issue (3): 377-382    DOI: 10.12891/ceog4617.2019
Original Research Previous articles | Next articles
The effect of VEGF and Ang-1 on cryopreserved human ovarian grafts in severe combined immunodeficient mice
Yin Shao1, Liguo Ma1, Meiyi Chen1, *(), Yuxia Guo1, Hongtao Xiao1
1Department of Gynecology, Shenzhen People's Hospital, Second Affiliated Hospital of Jinan University, Shenzhen, China
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Abstract  

This study investigates the independent and combined effect of vascular epithelial growth factor (VEGF) and angiopoietin 1 (Ang-1) on the follicle survival and vasculogenesis of cryopreserved human ovarian grafts in severe combined immunodeficient (SCID) mice. Cryopreserved human ovarian tissue was transplanted into the thigh of SCID mice with treatment of VEGF, Ang-1, or combination at the site of transplantation. Number of follicles, morphology change, follicle apoptosis, micro-vessel density (MVD), and expression of follicle development related genes were assessed in each transplanted ovarian group of SCID mice. VEGF and Ang-1 treatments increased functional follicles and MVD value, reduced the level of follicle apoptosis, and modulated expression of ovarian development related genes and FSH level. Synergistic effect of VEGF and Ang-1 on follicle survival was significantly stronger than treatment alone. In conclusion, treatment of VEGF and Ang-1 significantly improved the survival of follicles and vasculogenesis of transplanted human ovarian tissue in SCID mice.

Key words:  VEGF      Ang-1      Cryopreservation      Ovarian      Hetero-transplantation      Survival of follicles      Vasculogenesis     
Published:  10 June 2019     
*Corresponding Author(s):  MEIYI CHEN     E-mail:  doctorsy0755@163.com

Cite this article: 

Yin Shao, Liguo Ma, Meiyi Chen, Yuxia Guo, Hongtao Xiao. The effect of VEGF and Ang-1 on cryopreserved human ovarian grafts in severe combined immunodeficient mice. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(3): 377-382.

URL: 

https://ceog.imrpress.com/EN/10.12891/ceog4617.2019     OR     https://ceog.imrpress.com/EN/Y2019/V46/I3/377

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