Please wait a minute...
Clinical and Experimental Obstetrics & Gynecology  2019, Vol. 46 Issue (2): 280-284    DOI: 10.12891/ceog4518.2019
Original Research Previous articles | Next articles
Tspan5 inhibits proliferation and migration of JEG-3 cells by inhibiting FAK and AKT phosphorylation
X. Ren1, †*(), H-Y. Tang2, †, J. Wu1, T-Y. Gao1, †, J. Li1
1Department of Reproductive Medicine, Guangdong No.2 Provincial People’s Hospital, Guangzhou, China;
2Department of Reproductive Medicine, Meizhou People's Hospital, Meizhou, Guangdong, China
Download:  PDF(533KB)  ( 53 ) Full text   ( 5 )
Export:  BibTeX | EndNote (RIS)      
Abstract  

Objectives: To study the effect of Tspan5 on the proliferation and migration of trophoblast cells. Materials and Methods: The authors silenced the Tspan5 expression in human choriocarcinoma cell line JEG-3 using RNAi, and then conducted the cell proliferation assay and scratch assay to detect the proliferation and migration of JEG-3 cells. Results: The present authors found that the JEG-3 cell proliferation and migration activity of interference group (Tspan5/1005) had decreased significantly. Tspan5/1005 moderately decreased FAK and AKT phosphorylation compared to the control group. In this study, the authors found that the proliferation and migration of choriocarcinoma cells were reduced after using RNAi technology to downregulate the expression level of Tspan5 expression. The level of Tspan5 expression was positively correlated with the proliferation and migration of choriocarcinoma cells. Conclusions: Tspan5 could play a biological role through the FAK/AKT signaling pathway.

Key words:  Tspan5      Human choriocarcinoma cell line JEG-3      Proliferation      Migration      FAK/AKT     
Published:  10 April 2019     
*Corresponding Author(s):  X. REN     E-mail:  renxinvictory@163.com
About author:  Contributed equally.

Cite this article: 

X. Ren, H-Y. Tang, J. Wu, T-Y. Gao, J. Li. Tspan5 inhibits proliferation and migration of JEG-3 cells by inhibiting FAK and AKT phosphorylation. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(2): 280-284.

URL: 

https://ceog.imrpress.com/EN/10.12891/ceog4518.2019     OR     https://ceog.imrpress.com/EN/Y2019/V46/I2/280

[1] Shan Zhou, Liang Xia, Liyuan Han. SFRP1 suppresses granulosa cell proliferation and migration through inhibiting JNK pathway[J]. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(5): 1193-1199.
[2] M. Capell-Morell, N. Rodriguez-Mias, M. Cubo-Abert, M. Bradbury, J.L. Poza-Barrasus, A. Gil-Moreno. Removal of a migrated subdermal contraceptive implant: a case report and review of the literature[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(6): 965-967.
[3] G. Zhu, S.J. Liu, W. Lv. Bilateral borderline ovarian cystadenoma with migration of intrauterine device to right ovary accompanied with endometrial carcinoma in situ: a case report[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(5): 781-784.
[4] G.-Y. Xia, Q.-Q. Fu, H.-M. Li, M. Fang, T. Zhang, M.-W. Wu, L.-M. Chen, C.-Y. Wu, B. Yu, H.-T. Pan, X.-L. Shi. Downregulation of Hsp27 inhibits proliferation, migration and invasion in human choriocarcinoma cell line JAR[J]. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(2): 245-249.
[5] H. Jang, E.K. Park, D.S. Han, C.J. Kim, Y.S. Lee. Bladder stone formation on permanent suture material ten years after laparoscopic high uterosacral ligament suspension[J]. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(3): 472-474.
[6] L. Wang, Y. Li, X.P. Zhao, W.H. Zhang, W. Bai, Y.G. He. Hydronephrosis caused by intrauterine contraceptive device migration: three case reports with literature review[J]. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(2): 301-304.
[7] Y.B. Whang, S.Y. Kim, S.Y. Nam, S.G. Yeo, D.C. Park. Ectopic pregnancy occurring in the remnant tube of a previous adnexectomy: a case report[J]. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(1): 146-147.
[8] M. Adıyeke, M. Sancı, İ. Karaca, M. Gökçü, E. Töz, E. Öcal. Surgical management of intrauterine devices migrated towards intra-abdominal structures: 20-year experience of a tertiary center[J]. Clinical and Experimental Obstetrics & Gynecology, 2015, 42(3): 358-360.
[9] N. Vrachnis, N. Salakos, C. Iavazzo, Z. Iliodromiti, K. Bakalianou, P. Kouiroukidou, G. Creatsas. Female genital mutilation in Greece[J]. Clinical and Experimental Obstetrics & Gynecology, 2012, 39(3): 346-350.
[10] N. Ohara. Action of progesterone receptor modulators on uterine leiomyomas[J]. Clinical and Experimental Obstetrics & Gynecology, 2008, 35(3): 165-166.
[11] R. Corosu, A. Fedeli, A. Rossetti, P. Mancino. Pregnancy in immigrant women[J]. Clinical and Experimental Obstetrics & Gynecology, 2006, 33(3): 169-173.
[12] P. Panagopoulos, G. Tsoukalos, A. Economou, M. Zikopoulos, I. Koutras, G. Petrakos, M. Pachakis. Delivery and immigration: the experience of a Greek Hospital[J]. Clinical and Experimental Obstetrics & Gynecology, 2005, 32(1): 55-57.
[13] A. O. Mueck, H. Seeger, D. Wallwiener. ‘Pulsed’ estradiol action can stimulate breast cancer cell proliferation[J]. Clinical and Experimental Obstetrics & Gynecology, 2004, 31(1): 23-24.
[14] C. Lippert, H. Seeger, D. Wallwiener, A. O. Moeck. Comparison of the effects of 17α-ethinylestradiol and 17β-estradiol on the proliferation of human breast cancer cells and human umbilical vein endothelial cells[J]. Clinical and Experimental Obstetrics & Gynecology, 2002, 29(2): 87-90.
[15] A. Azzena, F. Vasoin, P. Pellizzari, F. Quintieri, R. Angarano. A rare case of IUD tubal migration[J]. Clinical and Experimental Obstetrics & Gynecology, 1994, 21(4): 246-248.
No Suggested Reading articles found!