Please wait a minute...
Clinical and Experimental Obstetrics & Gynecology  2019, Vol. 46 Issue (1): 27-31    DOI: 10.12891/ceog4255.2019
Original Research Previous articles | Next articles
Vaginal microbiota composition as a diagnostic tool for bacterial vaginosis in pregnant Korean women
Kwan Young Oh1, *(), Seung Min Yoo2, Yong Hak Sohn3, Seong Sil Chang4
1 Department of Obstetrics and Gynaecology, School of Medicine, Eulji University, Dunsanseo-ro, Seo-gu, Daejeon, Republic of Korea
2 Department of Microbiology, School of Medicine, Eulji University, Dunsanseo-ro, Seo-gu, Daejeon, Republic of Korea
3 Department of Laboratory Medicine, School of Medicine, Eulji University, Dunsanseo-ro, Seo-gu, Daejeon, Republic of Korea
4 Department of Occupational and Environmental Medicine, School of Medicine, Eulji University, Dunsanseo-ro, Seo-gu, Daejeon, Republic of Korea
Download:  PDF(530KB)  ( 202 ) Full text   ( 8 )
Export:  BibTeX | EndNote (RIS)      
Abstract  
Objectives: To analyze the relative abundance of five Lactobacillus species, Gardnerella vaginalis, and Atopobium vaginae in the vaginal microbiota of pregnant Korean women to establish diagnostic criteria for bacterial vaginosis (BV). Materials and Methods: Pregnant Korean women under antenatal care at Eulji University Hospital were enrolled at 10–14 weeks gestation. Twelve women were diagnosed with BV, nine had intermediate flora, and 93 had normal flora determined by Nugent score. Vaginal samples were collected to determine the quantification of seven microorganisms by qPCR and compared among the three groups. Results: This study indicated that G. vaginalis, A. vaginae, and L. iners were significantly increased specifically in the BV group (p < 0.001). While L. crispatus was significantly decreased in the BV group (p < 0.001). The area under curve (AUC) values for receiver operator characteristic (ROC) analysis were 0.934, 0.918, 0.763, and 0.742 for G. vaginalis, A. vaginae, L. iners, and L. crispatus, respectively, suggesting that patients with a higher quantification of G. vaginalis and A. vaginae were more likely to be diagnosed with BV (91.7% and 83.3%, respectively). Conclusions: The quantification of G. vaginalis and A. vaginae may be more useful diagnostic marker for BV in pregnant Korean women than those of Lactobacillus species.
Key words:  Bacterial vaginosis      Intermediate flora      Lactobacillus crispatus      Lactobacillus iners      Gardnerella vaginalis      Atopobium vaginae     
Published:  10 February 2019     
*Corresponding Author(s):  KWAN YOUNG OH     E-mail:  ohky5@eulji.ac.kr

Cite this article: 

Kwan Young Oh, Seung Min Yoo, Yong Hak Sohn, Seong Sil Chang. Vaginal microbiota composition as a diagnostic tool for bacterial vaginosis in pregnant Korean women. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(1): 27-31.

URL: 

https://ceog.imrpress.com/EN/10.12891/ceog4255.2019     OR     https://ceog.imrpress.com/EN/Y2019/V46/I1/27

[1] Dalia Saad ElFeky, Rasha Assiri, Hanadi Bakhsh, Ruba Almubaraz, Afrah Aldhowayan, Shahad Ismail Alruwaili, Raghad Alomairy. Microbiological pattern of laboratory confirmed vaginal infections among Saudi women[J]. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(4): 929-934.
[2] A. Dupre, H.H. Alur, D.R. Friend. Proof of concept study of a novel bioadhesive clindamycin phosphate 2% vaginal gel to treat bacterial vaginosis[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(4): 516-518.
[3] S. Nayak, A. Avery, J. McLeod Griffiss, C.D. Charles, K.R. Culwell. A randomized placebo-controlled pilot study of the effect and duration of Amphora, a multipurpose vaginal pH regulator, on vaginal pH[J]. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(5): 736-742.
[4] P. Nyirjesy, M. Padula, J.L. Amprey. Effect of 2016 FDA guidance on study population and clinical response rates in patients with bacterial vaginosis: a phase 3 post hoc analysis[J]. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(4): 615-617.
[5] M.K. Figueiredo Facundo, C.R. de Souza Bezerra Sakano, C.R. Nogueira de Carvalho, A.M. de Oliveira Machado, N.M. de Góis Speck, J. Chamorro Lascasas Ribalta. Vaginal microbiota in asymptomatic Brazilian women with HIV[J]. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(5): 704-709.
[6] P. Korrovits, E. Lapp, R. Mändar. Couple-related factors of ART outcome[J]. Clinical and Experimental Obstetrics & Gynecology, 2016, 43(5): 747-750.
[7] N. Güdücü, G. Gönenç, H. İşçi, A. Başgül Yig˘iter, N. Başsüllü, İ. Dünder. Clinical importance of detection of bacterial vaginosis, trichomonas vaginalis, candida albicans and actinomyces in Papanicolaou smears[J]. Clinical and Experimental Obstetrics & Gynecology, 2012, 39(3): 333-336.
[8] E. Caliskan, Y. Cakiroglu, D. Dundar, E. Doger, S. Caliskan, S. Ozeren. Integrating cervical length measurement into routine antenatal screening and only emergency cerclage when indicated[J]. Clinical and Experimental Obstetrics & Gynecology, 2009, 36(1): 40-45.
[9] E. D. Hapsari, M. Hayashi, H. Matsuo. Clinical characteristics of vaginal discharge in bacterial vaginosis diagnosed by Nugent's criteria[J]. Clinical and Experimental Obstetrics & Gynecology, 2006, 33(1): 5-9.
No Suggested Reading articles found!