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Clinical and Experimental Obstetrics & Gynecology  2017, Vol. 44 Issue (1): 30-38    DOI: 10.12891/ceog3181.2017
Original Research Previous articles | Next articles
A comparative immunohistochemical investigation of the consequences of chorioamnionitis on the developing human fetal spleen
E. Papadopoulos1, *(), C. Nikolaidou1, A. Kotini2, T.E. Deftereou1, J. Venizelos1, P. Pavlidis3, N. Gkantsinikoudis1, N. Papadopoulos1, M. Lambropoulou1
1 Laboratories of Histology-Embryology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
Laboratories of Medical Physics, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
Laboratories of Forensic Medicine, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
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Abstract  
Introduction: The objective of this study was to determine the effects of chorioamnionitis on the extracellular matrix (ECM) structural glycoproteins of the developing human fetal spleen, and their influence on the haematopoiesis and spleen immune system compared to controls. Materials and Methods: After elective induced pregnancy termination due to chorioamnionitis or voluntary abortion, paraffin-embedded specimens from the spleen and respective fetal membranes of 90 fetuses were investigated by immunohistochemistry for presence of ECM structural glycoproteins, haematopoietic, and lymphoid cells. Conventional histological examination of the relative fetal membranes was performed. Results: The present results showed no quantitative variations in the expression of the ECM glycoproteins and haematopoietic lineages of the fetal spleen parenchyma at the end of first trimester (in both groups). At the second and third trimesters, acute chorioamnionitis showed a decreased number of the aforementioned proteins, with an increase of granulopoiesis and CD34 progenitor/stem haematopoietic cells. The immune system of the spleen during the third trimester demonstrated a decrease of both B and T lymphocytes, in comparison with controls. Conclusions: These results suggest that toxins and cytokines generated during chorioamnionitis, seem to influence ECM structural glycoproteins synthesis and release in fetal splenic parenchyma by reducing them, and probably cause further disorders of haematopoiesis and lymphopoiesis.
Key words:  Fetal spleen      Chorioamnionitis      Structural glycoproteins      Haematopoiesis      Lymphopoiesis     
Published:  10 February 2017     
*Corresponding Author(s):  N. PAPADOPOULOS     E-mail:  npapad@med.duth.gr

Cite this article: 

E. Papadopoulos, C. Nikolaidou, A. Kotini, T.E. Deftereou, J. Venizelos, P. Pavlidis, N. Gkantsinikoudis, N. Papadopoulos, M. Lambropoulou. A comparative immunohistochemical investigation of the consequences of chorioamnionitis on the developing human fetal spleen. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(1): 30-38.

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https://ceog.imrpress.com/EN/10.12891/ceog3181.2017     OR     https://ceog.imrpress.com/EN/Y2017/V44/I1/30

[1] Shu-Jun Chen, Xie-Xia Zheng, Hong-Xing Jin, Jian-Hua Chen, Ting-Feng He, Cui-E Chen. Can venous cord blood neutrophil to lymphocyte ratio and platelet to lymphocyte ratio predict early-onset sepsis in preterm infants?[J]. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(4): 828-834.
[2] G. Balciuniene, L. Jakubauskiene, G.S. Drasutiene, A. Meskauskiene, D. Ramasauskaite. The significance of amniotic fluid immunological analysis for the prediction of intrauterine infection[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(6): 810-813.
[3] L. Lamprou, G. Panagopoulou, N. Papadopoulos, C. Tsigalou, O. Pagonopoulou, M. Lambropoulou. Impact of chorioamnionitis and other intrauterine pathological conditions on human foetal cytoskeleton structural protein development: An immunohistochemical study[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(6): 856-861.
[4] D. Lu, X. Bao, Q. Wang. Increasd mRNA expression of TRAF-6 and MST-4 in the placenta of women with preterm premature rupture of membranes with histological chorioamnionitis[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(5): 749-754.
[5] Xianhu Fu, Xiaobo He, Daifei Sun, Qihui Fan, Bailei Zhang, Qian Wang, Yinfen Wang, Huili Chen. Histological chorioamnionitis could be predicted with high accuracy in preterm labor with intact membranes before delivery[J]. Clinical and Experimental Obstetrics & Gynecology, 2018, 45(5): 720-724.
[6] T. Fukami, M. Goto, S. Matsuoka, S. Nishijima-Sorano, A. Tohyama, H. Yamamoto, S. Nakamura, R. Matsuoka, H. Tsujioka, F. Eguchi. Histologic chorioamnionitis prevalence in patients with premature rupture membranes[J]. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(2): 236-238.
[7] P. Boglou, T.H.-E. Deftereou, M. Lambropoulou, M. Katotomichelakis, V. Lambropoulou, O. Pagonopoulou, P. Chatzipantelis, N. Gkantsinikoudis, N. Papadopoulos, T.H. Dimitriou. Impact of chorioamnionitis on the development of human fetal lung: an immunohistochemical study[J]. Clinical and Experimental Obstetrics & Gynecology, 2015, 42(4): 457-461.
[8] M. Gojnic, A. Fazlagic, M. Pervulov, S. Petkovic, T. Mostic, K. Jeremie. The significance of C-reactive protein in the diagnosis of fetal tachycardia and therapy of chorioamnionitis[J]. Clinical and Experimental Obstetrics & Gynecology, 2005, 32(2): 114-116.
[9] J. P. Geisler, K. M. Horlander, A. K. Hiett. Methicillin resistant Staphylococcus aureus as a cause of chorioamnionitis[J]. Clinical and Experimental Obstetrics & Gynecology, 1998, 25(4): 119-120.
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