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Clinical and Experimental Obstetrics & Gynecology  2017, Vol. 44 Issue (1): 122-128    DOI: 10.12891/ceog3264.2017
Experimental Research Previous articles | Next articles
The effects of formoterol on the serum, peritoneal VEGF, MDA, and VEGF levels in the ovaries and endometrium of rats with OHSS
H.A. Inal1, *(), Z.H. Ozturk Inal1, N. Yilmaz2, H. Timur2, A.S. Oruc2, M.N. Kalem3, O. Han4
1 Konya Education and Research Hospital, Konya, Turkey
2 Dr. Zekai Tahir Burak Women’s Health Research and Education Hospital, Ankara, Turkey
3 Turgut Ozal University, Ankara, Turkey
4 Guven Hospital, Ankara, Turkey
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Abstract  
Purpose: To investigate the effects of formoterol (a beta2-adrenoreceptor agonist) on serum and peritoneal fluid VEGF, malondialdehyde (MDA) levels, and on VEGF-stained cell counts in the ovaries and endometrium of rats with ovarian hyperstimulation syndrome (OHSS) within the framework of immunohistochemical analysis. Materials and Methods: A total of 28 immature female Wistar rats were randomly divided into four groups. Three groups were given ten IU pregnant mare serum gonadotropin/day on days 22–25 of life. They were administered 30 IU hCG on day 26 of life to mimic OHSS. On days 26 and 27 of life, 24 mcg/kg/day formoterol in group 3 and 48 mcg/kg formoterol in group 4 were administered intraperitoneally per animal. Results: Although, there were no statistically significant differences between the groups in terms of serum and peritoneal fluid VEGF or MDA levels (serum VEGF: p = 0.281, peritoneal VEGF: p = 0.674, serum MDA: p = 0.543, peritoneal MDA: p = 0.506), there was a significant difference between the control and the OHSS placebo groups (p = 0.013) regarding the VEGF in the ovarian cortex. There was a significant difference between the control and the other groups in terms of ovarian stroma (p = 0.001), and there was also a statistically significant difference between the OHSS placebo and the other groups regarding VEGF in the endometrium (OHSS placebo vs. control group p = 0.002, OHSS placebo vs. the formoterol 24 mcg/kg group, p = 0.008, and OHSS-placebo vs. the formoterol 48 mcg/kg group, p = 0.001). Conclusions: Formoterol represents a potential novel strategy for the management of OHSS. Further studies, including those examining the dosage of formoterol, are warranted.
Key words:  OHSS      Prevention      VEGF      MDA      Formoterol     
Published:  10 February 2017     
*Corresponding Author(s):  H.A. INAL     E-mail:  dr.hasanaliinal@yahoo.com

Cite this article: 

H.A. Inal, Z.H. Ozturk Inal, N. Yilmaz, H. Timur, A.S. Oruc, M.N. Kalem, O. Han. The effects of formoterol on the serum, peritoneal VEGF, MDA, and VEGF levels in the ovaries and endometrium of rats with OHSS. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(1): 122-128.

URL: 

https://ceog.imrpress.com/EN/10.12891/ceog3264.2017     OR     https://ceog.imrpress.com/EN/Y2017/V44/I1/122

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