Please wait a minute...
Clinical and Experimental Obstetrics & Gynecology  2021, Vol. 48 Issue (5): 1094-1098    DOI: 10.31083/j.ceog4805176
Original Research Previous articles | Next articles
Defective expression of endometrial BMP-2 contributes to subfertility in women with unexplained infertility
Arzu Yurci1, *(), Nur Dokuzeylul Gungor2, Tugba Gurbuz3
1Gynecology Obstetrics & Reproductive Medicine, Memorial Kayseri Hospital IVF Center, 38110 Kayseri, Turkey
2Bahcesehir University Goztepe Medical Park Hospital, Gynecology Obstetrics & Reproductive Medicine, 34363 Istanbul, Turkey
3Medistate Hospital Gynecology and Obstetric Clinic, 34363 Istanbul, Turkey
Download:  PDF(116KB)  ( 57 ) Full text   ( 13 )
Export:  BibTeX | EndNote (RIS)      
Abstract  
Background: This study was planned to measure bone morphogenetic protein 2 (BMP-2) levels in the endometrial samples of unexplained infertility (UEI) cases who had a history of unsuccessful in vitro fertilization - embryo transfer (IVF-ET) and therefore decided to have frozen-thawed embryo transfer (FET). Methods: Thirty patients diagnosed with UEI and decided to transfer frozen-thawed embryo transfer were included in the study. The UEI patients were selected among the patients whose previous IVF-ET trials were unsuccessful and were scheduled for FET this time. Thirty participants in the control group were selected among patients diagnosed with polycystic ovary syndrome (PCOS) and scheduled for FET. Serum total testosterone, fasting insulin, follicle-stimulating hormone and luteinizing hormone levels were measured. Homeostatic model assessment - insulin resistance (HOMA-IR) Formula was used for calculating insulin resistance. Endometrial samples were collected by pipelle during oocyte pick-up, washed three times with a sterile saline solution to remove blood and transferred into RNA stabilization buffer until analysis. Endometrial BMP-2 concentrations were measured by enzyme-linked immunosorbent assay. Results: Serum total testosterone, insulin levels and HOMA-IR of patients in the control group were significantly higher than the UEI group. BMP-2 levels in the endometrial supernatants of UEI patients were found to be about 2 times lower than the patients in the control group (984.5 (350) pg/mg wet tissue vs. 1720 (318) pg/mg wet tissue, p < 0.000, Z = –6.6). A strong and significant positive correlation was found between endometrial thickness, estradiol levels measured on HCG day and BMP-2 levels. A positive and significant correlation was found between endometrial BMP-2 levels and serum luteinizing hormone (LH), fasting insulin, glucose, testosterone, HOMA-IR and mature oocyte counts. Conclusion: BMP-2 is associated with UEI and mechanistically it may be useful to study this further to determine is this is causal or merely a biomarker.
Key words:  Unexplained infertility      Endometrium      BMP-2      Subfertility     
Submitted:  09 January 2021      Revised:  13 February 2021      Accepted:  05 March 2021      Published:  15 October 2021     
*Corresponding Author(s):  Arzu Yurci     E-mail:  arzuyurci2@gmail.com

Cite this article: 

Arzu Yurci, Nur Dokuzeylul Gungor, Tugba Gurbuz. Defective expression of endometrial BMP-2 contributes to subfertility in women with unexplained infertility. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(5): 1094-1098.

URL: 

https://ceog.imrpress.com/EN/10.31083/j.ceog4805176     OR     https://ceog.imrpress.com/EN/Y2021/V48/I5/1094

[1] Zhongying Huang, Zhun Xiao, Qianhong Ma, Yu Bai, Feilang Li. Efficacy of tamoxifen for infertile women with thin endometrium undergoing frozen embryo transfer: a meta-analysis[J]. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(4): 806-811.
[2] A. Yaylalı, M. Bakacak, Z. Bakacak, F. Tolun. An investigation of the effects on follicular-embryonal development and pregnancy outcomes of serum and follicular fluid ischemia-modified albumin in cases of unexplained infertility receiving in vitro fertilization treatment[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(6): 835-839.
[3] J.H. Check, J. Liss, D. Check. The beneficial effect of luteal phase support on pregnancy rates in women with unexplained infertility[J]. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(3): 447-449.
[4] Chunjuan Shen, Liping Wang, Xiaojie Wu, Shuhui Mao, Chunxia Fang. The relationship between vitamin D and IVF: a systematic review and meta-analysis[J]. Clinical and Experimental Obstetrics & Gynecology, 2019, 46(1): 12-15.
[5] S.A. Hebeisha, F.S. Moiety, M. Samir, M. Hussein. Effect of endometrial injury on implantation and pregnancy rates: a randomised controlled trial[J]. Clinical and Experimental Obstetrics & Gynecology, 2018, 45(1): 105-108.
[6] J.H. Check. A second case of successful conception in a natural cycle despite a maximum endometrial thickness in the follicular phase of four mm[J]. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(3): 341-342.
[7] E. S. Güngör, C. Dane. Evaluation of ten years of intrauterine insemination results at a tertiary center[J]. Clinical and Experimental Obstetrics & Gynecology, 2016, 43(5): 759-762.
[8] J.H. Check, R. Cohen, J.K. Choe. Failure to improve a thin endometrium in the late proliferative phase with uterine infusion of granulocyte-colony stimulating factor[J]. Clinical and Experimental Obstetrics & Gynecology, 2014, 41(4): 473-475.
[9] L. Karakoc Sokmensuer, B. Demir, D. Zeybek, E. Asan, S. Gunalp. Human spermatozoa antigens in unexplained infertility[J]. Clinical and Experimental Obstetrics & Gynecology, 2013, 40(4): 475-481.
[10] J.H. Check, J. Liss, R. Cohen. A comparison of three types of therapies for three different ovulation disorders in establishing pregnancies and evaluation of laboratory parameters that could influence the outcome[J]. Clinical and Experimental Obstetrics & Gynecology, 2013, 40(3): 317-318.
[11] F. Kouakou, V. Loué, A. Kouamé, R. Adjoby, S. Koui, H. Koimé, E. Gbary. Endometrial osseous metaplasia and infertility: a case report[J]. Clinical and Experimental Obstetrics & Gynecology, 2012, 39(4): 559-561.
[12] J. Amui, J. H. Check, R. Cohen. Successful twin pregnancy in a donor oocyte recipientdespite a maximum endometrial thickness in the late proliferative phase of 4 mm[J]. Clinical and Experimental Obstetrics & Gynecology, 2011, 38(4): 328-329.
[13] J. Amui, J. H. Check, R. Cohen. Live fetus following embryo transfer in a womanwith diminished egg reserve whose maximal endometrial thickness was less than 4 mm[J]. Clinical and Experimental Obstetrics & Gynecology, 2011, 38(4): 330-332.
[14] J.H. Check, W. Yuan, M.C. Garberi-Levito, K. Swenson, K. McMonagle. Effect of method of oocyte fertilization on fertilization, pregnancy and implantation rates in women with unexplained infertility[J]. Clinical and Experimental Obstetrics & Gynecology, 2011, 38(3): 203-205.
[15] P. Iovenitti, G. Ruggeri, R. Tatangelo, P. Palermo, G. Carta. Endometrial tuberculosis: a clinical case[J]. Clinical and Experimental Obstetrics & Gynecology, 2011, 38(2): 186-187.
No Suggested Reading articles found!