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Clinical and Experimental Obstetrics & Gynecology  2021, Vol. 48 Issue (3): 483-486    DOI: 10.31083/j.ceog.2021.03.2259
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Diverse genetic characteristics of ESBL-producing Klebsiella pneumoniae isolates from obstetrics & gynaecology settings in some major hospitals, China
Xiao-Mei Zhang1, He Wang2, Ming-Cheng Li2, *, †(), Xue-Ying Bao1, †, Jia-Yu Chen3,
1Department of Clinical Laboratory, Associated Hospital, Beihua University, 132002 Jilin, Jilin, China
2Department of Molecular Diagnosis, School of Laboratory Medicine, Beihua University, 132013 Jilin, Jilin, China
3Department of Clinical Laboratory, Shaoxing University, 321000 Shaoxing, Zhejing, China
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Abstract  
Background: We investigated the susceptibility patterns and genotype of extended-spectrum-beta-lactamase (ESBL)-producing Klebsiella pneumonia isolates. Methods: A prospective survey of K. pneumoniae-infected patients was conducted in obstetrics & gynaecology settings in three associated hospitals of a university in China from 2017 to 2019. All isolates were identified as K. pneumoniae by the conventional standard procedures and confirmed by use of the VITEK GNI system. Susceptibility to 16 antimicrobial agents of ESBLs was determined by way of a screening procedure using discs. PCR amplification of bla genes, and sequencing of PCR products were performed to determine their molecular types. Results: A total of 518 of K. pneumoniae were isolated from sputum specimens in three affiliated hospitals. Out of these, 158 strains were ESBL producers and 64 strains were non-ESBL producers from the first hospital; 114 strains were positive for producing ESBLs and 60 strains were negative for ESBLs in the second hospital, and 86 strains were ESBL producer and 36 strains were negative from the third hospital. bla gene contents showed that 88 strains (24.6%) carried the blaTEM-2 gene, and 74 strains (20.1%) contained the blaSHV-3 gene, 44 strains (12.3%) had the blaCTX-M-2 gene, 42 strains (11.7%) had the blaCTX-M-4 gene, 38 strains (10.6%) harboured blaCTX-M-5 and blaCTX-M-8 genes, and 29 (9.5%) strains contained blaCTX-M-15 gene. Also, 20 (5.6%) strains contained blaOXA-1. The co-existence of blaCTX-M-15 and blaSHV-3 was identified in 20 strains (5.6%), 12 strains (3.3%) co-existed with blaSHV-3 and blaTEM-2, 14 isolates (3.9%) harboured blaTEM-2 andblaCTX-M-15, eight isolates (2.2%) possessed blaSHV-3, blaTEM-2, and blaCTX-M-15. Discussion: The high prevalence of ESBLs in K. pneumonia isolates with diverse enzyme gene types indicated the need for screening the changes in ESBL-producing isolates in this region.
Key words:  ESBLs      Klebsiella pneumoniae      Molecular epidemiology     
Submitted:  19 August 2020      Revised:  08 October 2020      Accepted:  21 October 2020      Published:  15 June 2021     
Fund: 
81402979/National Natural Science Foundation of China
81541141/National Natural Science Foundation of China
20140307008YY/Jilin and Zhejiang Science and Technology Development Program
2014C33155/Jilin and Zhejiang Science and Technology Development Program
2018J098/Health and Family Planning Commission of Jilin Province
*Corresponding Author(s):  limingcheng@beihua.edu.cn; baoxueying@beihua.edu.cn (Ming-Cheng Li)   
About author:  These authors contributed equally.

Cite this article: 

Xiao-Mei Zhang, He Wang, Ming-Cheng Li, Xue-Ying Bao, Jia-Yu Chen. Diverse genetic characteristics of ESBL-producing Klebsiella pneumoniae isolates from obstetrics & gynaecology settings in some major hospitals, China. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(3): 483-486.

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https://ceog.imrpress.com/EN/10.31083/j.ceog.2021.03.2259     OR     https://ceog.imrpress.com/EN/Y2021/V48/I3/483

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