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Clinical and Experimental Obstetrics & Gynecology  2021, Vol. 48 Issue (2): 416-422    DOI: 10.31083/j.ceog.2021.02.5455
Original Research Previous articles | Next articles
Expression of pelvic organ prolapse-related protein fibulin-5, TGFβ, and Smad2/3 in Uyghur women of Xinjiang
Shaadaiti Wufuer1, †, XiaoHui Wan1, †, Buhaiqiemu Kadeer1, Adilai Maimaitimin1, Gulina Ababaikeli1, *, †()
1Department of Gynecology, First Affiliated Hospital of Xinjiang Medical University, 830054 Urumqi, Xinjiang, China
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Abstract  
Objective: The purpose of this study was to investigate the role of fibulin-5 in the transforming growth factor beta (TGF-β)/Smad signaling pathway by measuring the differential expression of fibulin-5, TGF-β, and Smad2/3 in the connective tissue of the vaginal anterior wall of Uyghur women with pelvic organ prolapse (POP). Methods: Thirty-six Uyghur patients diagnosed with POP, who were admitted to the First Affiliated Hospital of Xinjiang Medical University from March 2015 to June 2018, were enrolled in the study. In the same period, 15 patients with benign uterine hysterectomy were included in the control group. The relative protein expression levels of fibulin-5, Smad2/3, TGF-β1, TGF-βRI, and TGF-βRII in the anterior vaginal wall of each group were determined by immunohistochemistry, quantitative PCR, and Western blotting. Results: Immunohistochemistry showed that the expression levels of Smad2/3, fibulin-5, TGF-β1, TGF-βRI, and TGF-βRII were significantly lower in the POP group than in the control group (P < 0.05). RT-PCR showed that the mRNA expression levels of TGF-β1, TGF-βRI, TGF-βRII, and fibulin-5 were significantly lower in the POP group, with statistical significance (P < 0.05), whereas the expression of Smad2 and Smad3 decreased, but without statistical significance (P > 0.05). Western blot analysis showed that the expression of TGF-β1, TGF-βRI, TGF-βRII, fibulin-5, and phosphorylated Smad2/3 was significantly lower in the POP group than in the control group (P < 0.05), but there was no difference in Smad2/3 protein expression compared with the control (P > 0.05). Conclusion: The amount of functional elastic fibers in the pelvic connective tissue structure of POP patients decreased via involvement of the TGF-β/Smad signaling pathway. Future studies are needed to confirm the pathogenesis of POP.
Key words:  Pelvic organ prolapse (POP)      Fibulin-5      TGF-β      Smad2/3      TGF-β/Smad signaling pathway     
Submitted:  27 March 2020      Revised:  17 September 2020      Accepted:  22 September 2020      Published:  15 April 2021     
Fund: 
81660252/National Natural Science Foundation of China (NSFC)
*Corresponding Author(s):  Gulina Ababaikeli     E-mail:  glnglna@163.com
About author:  These authors contributed equally.

Cite this article: 

Shaadaiti Wufuer, XiaoHui Wan, Buhaiqiemu Kadeer, Adilai Maimaitimin, Gulina Ababaikeli. Expression of pelvic organ prolapse-related protein fibulin-5, TGFβ, and Smad2/3 in Uyghur women of Xinjiang. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(2): 416-422.

URL: 

https://ceog.imrpress.com/EN/10.31083/j.ceog.2021.02.5455     OR     https://ceog.imrpress.com/EN/Y2021/V48/I2/416

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