Please wait a minute...
Clinical and Experimental Obstetrics & Gynecology  2021, Vol. 48 Issue (2): 283-291    DOI: 10.31083/j.ceog.2021.02.2267
Original Research Previous articles | Next articles
Maternal blood and amnionic oxytocin receptor gene expression and serum oxytocin levels in preterm birth: a case-control study
Kumari Anukriti1, *(), Kiran Guleria2, Vipin Tyagi3, Amita Suneja2, B D Banerjee3
1Department of Obstetrics and Gynaecology, AIIMS, 110029 Delhi, India
2Department of Obstetrics and Gynaecology, University College of Medical Sciences & G.T.B. Hospital (University of Delhi), Dilshad Garden, 110095 Delhi, India
3Environmental Biochemistry and Molecular Biology Laboratory, Department of Biochemistry, University College of Medical Sciences & G.T.B. Hospital (University of Delhi), Dilshad Garden, 110095 Delhi, India
Download:  PDF(1061KB)  ( 81 ) Full text   ( 9 )
Export:  BibTeX | EndNote (RIS)      
Abstract  
Purpose of investigation: The oxytocin (OXT)-oxytocin receptor (OXTR) system provides a promising candidate gene for studies of genetic contributions to prematurity. The author studies the quantification and comparison of oxytocin receptor (OXTR) gene expression and serum OXT levels in the blood and amnion of women delivering preterm and evaluation of the correlation between OXTR gene expression in blood and amnion with serum OXT levels in them. Material and methods: Seventy pregnant women in spontaneous labor delivering vaginally preterm i.e., < 37 weeks and an equal number of matched controls delivering spontaneously at term (37–42 weeks) were recruited. Maternal serum OXT levels were quantified by ELISA collected in the active stage of labor i.e., 4 cm cervical dilatation. Gene expression studies in the maternal blood and amnion were done by using real-time quantitative polymerase chain reaction (RT-qPCR). Results: The mean serum OXT level in preterm labor (PTL) was 48.56 ± 6.97 pg/mL; significantly higher than in controls (43.00 ± 3.96 pg/mL), P < 0.001. OXTR gene expression in maternal blood (2.5 times) as well as in amnion (3.5 times) was significantly higher in PTL. A significant positive correlation was observed between serum OXT levels and OXTR gene expression in amnion (r = -0.190, P = 0.025). Conclusions: The serum OXT levels and OXTR gene expression in amnion surge significantly in the active phase of PTL. Thus, amnion probably links OXT-PTGs (prostaglandins) autocrine paracrine circuit to facilitate PTL. Future studies are needed to devise better OXTR receptor antagonists preferably acting on amnionic OXTRs to prevent inflammatory pathways leading to PTL.
Key words:  Preterm birth      Preterm labor      Oxytocin      Oxytocin receptor      Placenta      Amnion     
Submitted:  22 August 2020      Revised:  26 December 2020      Accepted:  11 January 2021      Published:  15 April 2021     
Fund: 
Indian Council of Medical Research (ICMR)
*Corresponding Author(s):  Kumari Anukriti     E-mail:  anukritieyes@gmail.com

Cite this article: 

Kumari Anukriti, Kiran Guleria, Vipin Tyagi, Amita Suneja, B D Banerjee. Maternal blood and amnionic oxytocin receptor gene expression and serum oxytocin levels in preterm birth: a case-control study. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(2): 283-291.

URL: 

https://ceog.imrpress.com/EN/10.31083/j.ceog.2021.02.2267     OR     https://ceog.imrpress.com/EN/Y2021/V48/I2/283

[1] Yu Huang, Fang-Yuan Luo. Clinical efficacy of aortic balloon occlusion during caesarean section in patients with placenta accreta spectrum disorders: a systematic review and meta-analysis[J]. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(2): 234-244.
[2] Hyun Jin Ko, Seong Yeon Hong, Jin Young Bae. Pregnancy and neonatal outcomes of hyperglycemia caused by atosiban administration during pregnancy[J]. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(2): 257-262.
[3] Ryuichi Shimaoka, Tomomi Shiga, Ken-ichirou Morishige. Change in uterine artery blood flow with intrauterine balloon tamponade[J]. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(2): 307-311.
[4] Saša Raičević, Duško Kljakić, Filip Vukmirović, Miloš Z. Milosavljević. A giant placental chorioangioma with a resultant live birth; a discussion of management options[J]. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(2): 426-430.
[5] Suchaya Luewan, Pakorn Chaksuwat, Tip Pongsuvareeyakul, Theera Tongsong. Prenatal sonographic findings and management of placental mesenchymal dysplasia[J]. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(2): 439-443.
[6] Elisa Zambrotta, Luisa Maria Di Gregorio, Federica Di Guardo, Roberta Agliozzo, Giuliana Chiara Maugeri, Ferdinando Antonio Gulino, Silvia Cutello, Maria Cecilia Cerana, Marco Palumbo. Congenital uterine anomalies and perinatal outcomes: a retrospective single-center cohort study[J]. Clinical and Experimental Obstetrics & Gynecology, 2021, 48(1): 160-163.
[7] G. Balciuniene, L. Jakubauskiene, G.S. Drasutiene, A. Meskauskiene, D. Ramasauskaite. The significance of amniotic fluid immunological analysis for the prediction of intrauterine infection[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(6): 810-813.
[8] L. Lamprou, G. Panagopoulou, N. Papadopoulos, C. Tsigalou, O. Pagonopoulou, M. Lambropoulou. Impact of chorioamnionitis and other intrauterine pathological conditions on human foetal cytoskeleton structural protein development: An immunohistochemical study[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(6): 856-861.
[9] L.L. Xu, J.Q. Li, Y.Q. Pu, C. Zhou, S.W. Feng, Q. Luo. Effect of prenatal depression during late pregnancy on maternal and neonatal outcomes[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(5): 681-686.
[10] I.F. Urunsak, U.K. Gulec, E. Eser, M. Sucu, C. Akcabay, S. Buyukkurt. The role of dinoprostone for labor induction in postterm and high-risk term pregnancies[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(5): 664-668.
[11] N. Al-Husban, A. Hababeh, M. Al-Kasasbeh, R. Odeh, M. Shaheen, D. Moughrabi, D. Zabalawi. Thyroid stimulating hormone (TSH) level variations in early pregnancy and feto-maternal outcome; retrospective study[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(5): 675-680.
[12] S. Han, S. Choi, S. Nah, Y.H. Lee. Preterm labor in mild carbon monoxide poisoning: a case report[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(5): 805-806.
[13] D. Lu, X. Bao, Q. Wang. Increasd mRNA expression of TRAF-6 and MST-4 in the placenta of women with preterm premature rupture of membranes with histological chorioamnionitis[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(5): 749-754.
[14] H.M. Kim, Y.J. Chin, H.H. Cha, W.J. Seong, M.J. Kim. Placenta accreta and uterine rupture of unscarred uterus in patients with systemic lupus erythematosus with prolonged steroid exposure: a report of two cases[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(5): 785-788.
[15] G. Garuti, E. Castellacci, S. Calabrese, S. Calzolari. Hysteroscopic removal of retained products of conception with enhanced vascularity: a study of reliability[J]. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(4): 472-477.
[1] Eli M. Roth, Michael H. Davidson. PCSK9 Inhibitors: Mechanism of Action, Efficacy, and Safety[J]. Reviews in Cardiovascular Medicine, 2018, 19(S1): 31 -46 .
[2] Sandeep K. Krishnan, Norman E. Lepor. Acute and Chronic Cardiovascular Effects of Hyperkalemia: New Insights Into Prevention and Clinical Management[J]. Reviews in Cardiovascular Medicine, 2016, 17(S1): 9 -21 .
[3] Ibrahim Sidiqi, Patrick Alexander. Current Advances in Endovascular Therapy for Infrapopliteal Artery Disease[J]. Reviews in Cardiovascular Medicine, 2015, 16(1): 36 -50 .
[4] Peter Shalit. Management of Dyslipidemia in Patients With Human Immunodeficiency Virus[J]. Reviews in Cardiovascular Medicine, 2014, 15(S1): 38 -46 .
[5] Sophie Mavrogeni, Fabrizio Cantini, Gerald M. Pohost. Systemic Vasculitis: An Underestimated Cause of Heart Failure—Assessment by Cardiovascular Magnetic Resonance[J]. Reviews in Cardiovascular Medicine, 2013, 14(1): 49 -55 .
[6] George L. Smith. Appropriate Use Criteria: The Gold Standard, or a Mechanism for the Derogation of Clinical Judgment?[J]. Reviews in Cardiovascular Medicine, 2011, 12(2): 105 .
[7] Jeffrey W. Moses, Stephane Carlier, Issam Moussa. Lesion Preparation Prior to Stenting[J]. Reviews in Cardiovascular Medicine, 2004, 5(S2): 16 -21 .
[8] Dean J. Kereiakes. Coronary Small-Vessel Stenting in the Era of Drug Elution[J]. Reviews in Cardiovascular Medicine, 2004, 5(S2): 34 -45 .
[9] Steven V. Edelman. The Role of the Thiazolidinediones in the Practical Management of Patients With Type 2 Diabetes and Cardiovascular Risk Factors[J]. Reviews in Cardiovascular Medicine, 2003, 4(S6): 29 -37 .
[10] M. Pakiž, L. Lukman, N. Kozar. Patients' and physicians' expectations differ significantly during the follow-up period after completion of primary treatment of gynecological or breast cancer[J]. European Journal of Gynaecological Oncology, 2019, 40(5): 781 -786 .