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Clinical and Experimental Obstetrics & Gynecology  2020, Vol. 47 Issue (5): 764-768    DOI: 10.31083/j.ceog.2020.05.5228
Original Research Previous articles | Next articles
Effect of extended embryo culture after thawing on clinical pregnancy rate
Sh. Gaafar1, *(), T. Hanafy1, S. El Morshedy2, H. Mansour1
1Obstetrics and Gynaecology Department, Faculty of Medicine, Alexandria University, Alexandria, 21221, Egypt
2Obstetrics and Gynaecology Department, Dar Ismael hospital, Alexandria, 21245, Egypt
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Abstract  
Purpose: This randomized controlled trial is aiming to compare the clinical pregnancy rate of immediate versus delayed post thawing embryo transfer in frozen embryo transfer (FET) cycles. Materials and Methods: The study was conducted on 150 FET cycles in which embryos were vitrified on day 2 or 3. After thawing, cases with more than 50% blastomere loss were excluded as well as cases with recurrent implantation. The endometrium was prepared by programmed estrogen/progesterone method. After thawing, cases were randomized to immediate embryo transfer or extended culture for 18-72 hours before transfer. Results: Number of cryopreserved embryos and survival rates were similar in both groups. Survival rates was above 95% in both groups and in the extended culture group 72.1% of embryos resumed miosis. Clinical pregnancy rate was 50.7% in immediate transfer group and 41.3% in the extended culture group. Conclusion: Extended culture after thawing helps in embryo selection but does not increase clinical pregnancy rate.
Key words:  ICSI      Frozen embryo transfer      Thawing      Extended culture      Blastocyst transfer     
Submitted:  20 April 2019      Accepted:  15 July 2019      Published:  15 October 2020     
*Corresponding Author(s):  SHERIF SALAH GAAFAR     E-mail:  shgaafar301@yahoo.com

Cite this article: 

Sh. Gaafar, T. Hanafy, S. El Morshedy, H. Mansour. Effect of extended embryo culture after thawing on clinical pregnancy rate. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(5): 764-768.

URL: 

https://ceog.imrpress.com/EN/10.31083/j.ceog.2020.05.5228     OR     https://ceog.imrpress.com/EN/Y2020/V47/I5/764

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