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Clinical and Experimental Obstetrics & Gynecology  2020, Vol. 47 Issue (4): 546-551    DOI: 10.31083/j.ceog.2020.04.5144
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Dietary sesame diminishes bone mass and bone formation indices in ovariectomized rats
R. Tachibana1, H. Matsushita1, *(), A. Minami2, N. Morita1, S. Shimizu1, H. Kanazawa3, T. Suzuki2, K. Watanabe1, A. Wakatsuki1
1Department of Obstetrics and Gynecology, School of Medicine, Aichi Medical University, Nagakute, Aichi, Japan
2Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
3Department of Functional Anatomy, School of Nursing, University of Shizuoka, Shizuoka, Japan
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Abstract  

Purpose: This animal experiment investigated how sesame seed (Sesamum indicum; SSI) extract affects bone metabolism in ovariectomized (Ovx) rats. Materials and methods: Female Wistar rats (12 weeks old) were randomly assigned to the Baseline control (BC), Sham, Ovx, and Ovx + SSI groups (n = 6-10). Animals in the BC group were sacrificed immediately. Rats in the other groups underwent sham surgery (Sham) or bilateral ovariectomy (Ovx and Ovx + SSI). Rats in the Ovx + SSI group were given custom diets, and BMD and bone histomorphometry were evaluated 13 weeks post-surgery. Results: The femur BMD in the Ovx + SSI group was lower than in the Ovx group, as were the measurements for bone formation rate and mineralizing surface. Conclusion: Administration of a methanol extract of sesame seeds decreased femur BMD in Ovx rats.

Key words:  Bone mineral density      Bone histomorphometry      Menopause      Ovariectomy      Rat      Sesame     
Submitted:  01 February 2019      Accepted:  01 April 2019      Published:  15 August 2020     
Fund: Japan Society for Menopause and Women's Health to A.M.
*Corresponding Author(s):  H. Matsushita     E-mail:  hirosm@aichi-med-u.ac.jp

Cite this article: 

R. Tachibana, H. Matsushita, A. Minami, N. Morita, S. Shimizu, H. Kanazawa, T. Suzuki, K. Watanabe, A. Wakatsuki. Dietary sesame diminishes bone mass and bone formation indices in ovariectomized rats. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(4): 546-551.

URL: 

https://ceog.imrpress.com/EN/10.31083/j.ceog.2020.04.5144     OR     https://ceog.imrpress.com/EN/Y2020/V47/I4/546

Figure 1.  — BMD of the whole femur (A) and the proximal (B), middle (C) and distal (D) thirds of the femur of the BC, Sham, Ovx and Ovx + SSI group. Values are expressed as the mean ± standard error the mean. Data for BC, Sham, and Ovx groups are taken from Morita et al. [20]. aP < 0.01 vs. Sham group, and bP < 0.05 and cP < 0.01 vs. Ovx group (one-way ANCOVA with Tukey’s honest significant difference test). BMD, bone mineral density; BC, baseline control; SSI, Sesamum indicum; ANCOVA, analysis of covariance.

Table 1  — Structural cancellous bone indices measured at the proximal tibial metaphysis of the BC, Sham, Ovx and Ovx + SSI group.
BCa Shama Ovxa Ovx + SSI
Structural indices
Bone volume, % 21.7 ± 0.9 29.7 ± 1.7 8.0 ± 0.8c 8.9 ± 0.9c
Trabecular thickness, μm 58.6 ± 1.8 69.7 ± 3.0 61.8 ± 1.4 63.2 ± 1.6
Trabecular number, /mm 3.69 ± 0.09 4.25 ± 0.12 1.29 ± 0.10c 1.40 ± 0.13c
Osteoid surface, % 23.1 ± 2.1 18.5 ± 2.1 31.8 ± 2.3c 28.6 ± 2.0c
Osteoid volume, % 3.00 ± 0.34 2.11 ± 0.33 4.28 ± 0.49c 3.32 ± 0.27b
Osteoblast surface, % 3.47 ± 0.44 1.13 ± 0.25 3.15 ± 0.65c 3.17 ± 0.52c
Eroded surface, % 7.51 ± 1.02 3.32 ± 0.16 4.04 ± 0.35 5.13 ± 0.77
Osteoclast surface, % 4.64 ± 0.69 0.65 ± 0.11 1.29 ± 0.12 1.86 ± 0.28c
Figure 2.  — Dynamic indices of cancellous bone at the proximal tibiae of the BC, Sham, Ovx and Ovx + SSI group. Values are expressed as the mean ± standard error the mean. Data for BC, Sham, and Ovx groups are taken from Morita et al. [20]. aP < 0.05 and bP < 0.01 vs. Sham group, and cP < 0.05 and dP < 0.01 compared vs. Ovx group (one-way ANCOVA with Tukey’s honest significant difference test). BC, baseline control; SSI, Sesamum indicum; ANCOVA, analysis of covariance.

[1] NIH Consensus Development Panel on Osteoporosis Prevention, Diagnosis, and Therapy: “Osteoporosis prevention, diagnosis, and therapy”. JAMA, 2001, 285, 785.
doi: 10.1001/jama.285.6.785 pmid: 11176917
[2] Yoshimura N., Muraki S., Oka H., Mabuchi A., En-Yo Y., Yoshida M., et al.: “Prevalence of knee osteoarthritis, lumbar spondylosis, and osteoporosis in Japanese men and women: the research on osteoarthritis/osteoporosis against disability study”. J. Bone Miner. Metab., 2009, 27, 620.
doi: 10.1007/s00774-009-0080-8
[3] Raisz L.G.: “Pathogenesis of osteoporosis: Concepts, conflicts, and prospects”. J. Clin. Invest., 2005, 115, 3318.
doi: 10.1172/JCI27071 pmid: 16322775
[4] Wells G., Tugwell P., Shea B., Guyatt G., Peterson J., Zytaruk N., et al.: “Meta-analysis of the efficacy of hormone replacement therapy in treating and preventing osteoporosis in postmenopausal women”. Endocr. Rev., 2002, 3, 529.
[5] Dören M., Nilsson J.A., Johnell O.: “Effects of specific post-menopausal hormone therapies on bone mineral density in post-menopausal women: A meta-analysis”. Hum. Reprod., 2003, 18, 1737.
doi: 10.1093/humrep/deg315 pmid: 12871893
[6] Torgerson D.J., Bell-Syer S.E.: “Hormone replacement therapy and prevention of nonvertebral fractures: A meta-analysis of randomized trials”. JAMA, 2001, 285, 2891.
doi: 10.1001/jama.285.22.2891 pmid: 11401611
[7] Michaëlsson K., Baron J.A., Farahmand B.Y., Johnell O., Magnusson C., Persson P.G., Persson I., Ljunghall S.: “Hormone replacement therapy and risk of hip fracturs: population based case-control study. The Swedish Hip Fracture Study Group”. BMJ, 1998, 316, 1858.
doi: 10.1136/bmj.316.7148.1858 pmid: 9632404
[8] Randell K.M., Honkanen R.J., Kröger H., Saarikoski S.: “Does hormone-replacement therapy prevent fractures in early postmenopausal women?”. J. Bone Miner. Res., 2002, 17, 528.
doi: 10.1359/jbmr.2002.17.3.528 pmid: 11874244
[9] Cauley J.A., Seeley D.G., Ensrud K., Ettinger B., Black D., Cummings S.R.: “Estrogen replacement therapy and fractures in older women. Study of Osteoporotic Fractures Research Group”. Ann. Intern. Med., 1995, 122, 9.
[10] Naessén T., Persson I., Adami H.O., Bergström R., Bergkvist L.: “Hormone replacement therapy and the risk for first hip fracture. A prospective, population-based cohort study”. Ann. Intern. Med., 1990, 113, 95.
doi: 10.7326/0003-4819-113-2-95 pmid: 2360759
[11] Rossouw J.E, Anderson G.L., Prentice R.L., LaCroix A.Z., Kooperberg C., Stefanick M.L., et al.: “Risks and benefits of estrogen plus progestin in healthy postmenopausal women: Principal results From the Women's Health Initiative randomized controlled trial”. JAMA, 2002, 288, 321
doi: 10.1001/jama.288.3.321 pmid: 12117397
[12] Camacho P.M., Petak S.M., Binkley N., Clarke B.L., Harris S.T., Hurley D.L., et al.: “American association of clinical endocrinologists and american college of endocrinology clinical practice guidelines for the diagnosis and treatment of postmenopausal osteoporosis - 2016”. Endocr. Pract., 2016, 22, 1.
doi: 10.4158/1530-891X-22.s2.1 pmid: 27206073
[13] Namiki M.: “The chemistry and physiological functions of sesame”. Food Rev. Int., 1995, 11, 281.
doi: 10.1080/87559129509541043
[14] Thompson L.U., Boucher B.A., Liu Z., Cotterchio M., Kreiger N.: “Phytoestrogen content of foods consumed in Canada, including isoflavones, lignans, and coumestan”.. Nutr Cancer, 2006, 54, 184.
[15] Mazur W.: “Phytoestrogen content in foods”. Bailliere’s Clin. Endocrinol. Metab., 1998, 12, 729.
doi: 10.1016/S0950-351X(98)80013-X
[16] Wu W.H.: “The contents of lignans in commercial sesame oils of Taiwan and their changes during heating”. Food Chem., 2007, 104, 341.
doi: 10.1016/j.foodchem.2006.11.055
[17] Liu Z., Saarinen N.M., Thompson L.U.: “Sesamin is one of the major precursors of mammalian lignans in sesame seed (Sesamum indicum) as observed in vitro and in rats”. J. Nutr., 2006, 136, 906.
doi: 10.1093/jn/136.4.906 pmid: 16549449
[18] Dar A.A., Arumugam N.: “Lignans of sesame: purification methods, biological activities and biosynthesis--a review”. Bioorg. Chem., 2013, 50, 1.
doi: 10.1016/j.bioorg.2013.06.009
[19] Peñalvo J.L., Heinonen S.M., Aura A.M., Adlercreutz H.: “Dietary sesamin is converted to enterolactone in humans”. J. Nutr., 2005, 135, 1056.
doi: 10.1093/jn/135.5.1056 pmid: 15867281
[20] Morita N., Matsushita H., Minami A., Shimizu S., Tachibana R., Kanazawa H., et al.: “Effect of the methanol extract of the Lion’s Mane mushroom, Hericium erinacenus, on bone metabolism in ovariectomized rats”. Clin. Exp. Obstet. Gynecol., 2020, 47, 47-52.
doi: 10.31083/j.ceog.2020.01.4987
[21] Kalu D.N.: “The ovariectomized rat model of postmenopausal bone loss”. Bone Miner., 1991, 15, 175.
doi: 10.1016/0169-6009(91)90124-i pmid: 1773131
[22] Dempster D.W., Compston J.E., Drezner M.K., Glorieux F.H., Kanis J.A., Malluche H., et al.: “Standardized nomenclature, symbols, and units for bone histomorphometry: A 2012 update of the report of the ASBMR Histomorphometry Nomenclature Committee”. J. Bone Miner. Res.,2013, 28, 2.
doi: 10.1002/jbmr.1805 pmid: 23197339
[23] Lerner U.H.: “Bone remodeling in post-menopausal osteoporosis”. J. Dent. Res., 2006, 85, 584.
doi: 10.1177/154405910608500703 pmid: 16798857
[24] Clarke B.: “Normal bone anatomy and physiology”. Clin. J. Am. Soc. Nephrol., 2008, 3 Suppl 3, S131.
doi: 10.2215/CJN.04151206 pmid: 18988698
[25] Boulbaroud S., Mesfioui A., Arfaoui A., Ouichou A., El Hessni A.: “Preventive effects of flaxseed and sesame oil on bone loss in ovariectomized rats”. Pak. J. Biol. Sci., 2008, 11, 1969.
doi: 10.3923/pjbs.2008.1969.1978 pmid: 19266902
[26] Hassan H.A., El Wakf A.M., El Gharib N.E.: “Role of phytoestrogenic oils in alleviating osteoporosis associated with ovariectomy in rats”. Cytotechnology, 2013, 65, 609.
doi: 10.1007/s10616-012-9514-6
[27] Sacco S.M., Chen J., Power K.A., Ward W.E., Thompson L.U.: “Lignan-rich sesame seed negates the tumor-inhibitory effect of tamoxifen but maintains bone health in a postmenopausal athymic mouse model with estrogen-responsive breast tumors”. Menopause, 2008, 15, 171.
doi: 10.1097/gme.0b013e3180479901 pmid: 17545920
[28] Herrera E., Barbas C.: “Vitamin E: action, metabolism and perspectives”. J. Physiol. Biochem., 2001, 57, 43.
doi: 10.1007/BF03179812
[29] Stocker A.: “Molecular mechanisms of vitamin E transport”.. Ann. N. Y Acad. Sci., 2004, 1031, 44.
doi: 10.1196/annals.1331.005
[30] Yamashita K., Nohara Y., Katayama K., Namiki M.: “Sesame seed lignans and gamma-tocopherol act synergistically to produce vitamin E activity in rats”. J. Nutr., 1992, 122, 2440.
doi: 10.1093/jn/122.12.2440 pmid: 1453229
[31] Yamashita K., Iizuka Y., Imai T., Namiki M.: “Sesame seed and its lignans produce marked enhancement of vitamin E activity in rats fed a low alpha-tocopherol diet”. Lipids, 1995, 30, 1019.
doi: 10.1007/BF02536287 pmid: 8569430
[32] Sontag T.J., Parker R.S.: “Influence of major structural features of tocopherols and tocotrienols on their ω-oxidation by tocopherol-ω-hydroxylase”. J. Lipid Res., 2007, 48, 1090.
doi: 10.1194/jlr.M600514-JLR200 pmid: 17284776
[33] Sontag T.J., Parker R.S.: “Cytochrome P450 omega-hydroxylase pathway of tocopherol catabolism. Novel mechanism of regulation of vitamin E status”. J. Biol. Chem., 2002, 277, 25290.
doi: 10.1074/jbc.M201466200 pmid: 11997390
[34] Lemcke-Norojärvi M., Kamal-Eldin A., Appelqvist L.A., Dimberg L.H., Ohrvall M., Vessby B.: “Corn and sesame oils increase serum γ-tocopherol concentrations in healthy Swedish women”. J. Nutr., 2001, 131, 1195.
doi: 10.1093/jn/131.4.1195 pmid: 11285325
[35] Cooney R.V., Custer L.J., Okinaka L., Franke A.A.: “Effects of dietary sesame seeds on plasma tocopherol levels”.. Nutr Cancer, 2001, 39, 66.
doi: 10.1002/1097-0142(197701)39:1<66::aid-cncr2820390113>3.0.co;2-4 pmid: 832252
[36] Wu W.H., Kang Y.P., Wang N.H., Jou H.J., Wang T.A.: “Sesame ingestion affects sex hormones, antioxidant status, and blood lipids in postmenopausal women”. J. Nutr., 2006, 136, 1270.
doi: 10.1093/jn/136.5.1270 pmid: 16614415
[37] Frank J., Lee S., Leonard S.W., Atkinson J.K., Kamal-Eldin A., Traber M.G.: “Sex differences in the inhibition of gamma-tocopherol metabolism by a single dose of dietary sesame oil in healthy subjects”. Am. J. Clin. Nutr., 2008, 87, 1723.
doi: 10.1093/ajcn/87.6.1723 pmid: 18541561
[38] Hamidi M.S., Corey P.N., Cheung A.M.: “Effects of vitamin E on bone turnover markers among US postmenopausal women”. J. Bone Miner. Res., 2012, 27, 1368.
doi: 10.1002/jbmr.1566 pmid: 22308007
[39] Fujita K., Iwasaki M., Ochi H., Fukuda T., Ma C., Miyamoto T., et al.: “Vitamin E decreases bone mass by stimulating osteoclast fusion”. Nat. Med., 2012, 18, 589.
doi: 10.1038/nm.2659 pmid: 22388090
[40] Ikegami H., Kawawa R., Ichi I., Ishikawa T., Koike T., Aoki Y., et al.: “Excessive Vitamin E Intake Does Not Cause Bone Loss in Male or Ovariectomized Female Mice Fed Normal or High-Fat Diets”. J. Nutr., 2017, 147, 1932.
doi: 10.3945/jn.117.248575 pmid: 28835390
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