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Clinical and Experimental Obstetrics & Gynecology  2020, Vol. 47 Issue (4): 546-551    DOI: 10.31083/j.ceog.2020.04.5144
Original Research Previous articles | Next articles
Dietary sesame diminishes bone mass and bone formation indices in ovariectomized rats
R. Tachibana1, H. Matsushita1, *(), A. Minami2, N. Morita1, S. Shimizu1, H. Kanazawa3, T. Suzuki2, K. Watanabe1, A. Wakatsuki1
1Department of Obstetrics and Gynecology, School of Medicine, Aichi Medical University, Nagakute, Aichi, Japan
2Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
3Department of Functional Anatomy, School of Nursing, University of Shizuoka, Shizuoka, Japan
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Purpose: This animal experiment investigated how sesame seed (Sesamum indicum; SSI) extract affects bone metabolism in ovariectomized (Ovx) rats. Materials and methods: Female Wistar rats (12 weeks old) were randomly assigned to the Baseline control (BC), Sham, Ovx, and Ovx + SSI groups (n = 6-10). Animals in the BC group were sacrificed immediately. Rats in the other groups underwent sham surgery (Sham) or bilateral ovariectomy (Ovx and Ovx + SSI). Rats in the Ovx + SSI group were given custom diets, and BMD and bone histomorphometry were evaluated 13 weeks post-surgery. Results: The femur BMD in the Ovx + SSI group was lower than in the Ovx group, as were the measurements for bone formation rate and mineralizing surface. Conclusion: Administration of a methanol extract of sesame seeds decreased femur BMD in Ovx rats.

Key words:  Bone mineral density      Bone histomorphometry      Menopause      Ovariectomy      Rat      Sesame     
Submitted:  01 February 2019      Accepted:  01 April 2019      Published:  15 August 2020     
Fund: Japan Society for Menopause and Women's Health to A.M.
*Corresponding Author(s):  H. Matsushita     E-mail:

Cite this article: 

R. Tachibana, H. Matsushita, A. Minami, N. Morita, S. Shimizu, H. Kanazawa, T. Suzuki, K. Watanabe, A. Wakatsuki. Dietary sesame diminishes bone mass and bone formation indices in ovariectomized rats. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(4): 546-551.

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Figure 1.  — BMD of the whole femur (A) and the proximal (B), middle (C) and distal (D) thirds of the femur of the BC, Sham, Ovx and Ovx + SSI group. Values are expressed as the mean ± standard error the mean. Data for BC, Sham, and Ovx groups are taken from Morita et al. [20]. aP < 0.01 vs. Sham group, and bP < 0.05 and cP < 0.01 vs. Ovx group (one-way ANCOVA with Tukey’s honest significant difference test). BMD, bone mineral density; BC, baseline control; SSI, Sesamum indicum; ANCOVA, analysis of covariance.

Table 1  — Structural cancellous bone indices measured at the proximal tibial metaphysis of the BC, Sham, Ovx and Ovx + SSI group.
BCa Shama Ovxa Ovx + SSI
Structural indices
Bone volume, % 21.7 ± 0.9 29.7 ± 1.7 8.0 ± 0.8c 8.9 ± 0.9c
Trabecular thickness, μm 58.6 ± 1.8 69.7 ± 3.0 61.8 ± 1.4 63.2 ± 1.6
Trabecular number, /mm 3.69 ± 0.09 4.25 ± 0.12 1.29 ± 0.10c 1.40 ± 0.13c
Osteoid surface, % 23.1 ± 2.1 18.5 ± 2.1 31.8 ± 2.3c 28.6 ± 2.0c
Osteoid volume, % 3.00 ± 0.34 2.11 ± 0.33 4.28 ± 0.49c 3.32 ± 0.27b
Osteoblast surface, % 3.47 ± 0.44 1.13 ± 0.25 3.15 ± 0.65c 3.17 ± 0.52c
Eroded surface, % 7.51 ± 1.02 3.32 ± 0.16 4.04 ± 0.35 5.13 ± 0.77
Osteoclast surface, % 4.64 ± 0.69 0.65 ± 0.11 1.29 ± 0.12 1.86 ± 0.28c
Figure 2.  — Dynamic indices of cancellous bone at the proximal tibiae of the BC, Sham, Ovx and Ovx + SSI group. Values are expressed as the mean ± standard error the mean. Data for BC, Sham, and Ovx groups are taken from Morita et al. [20]. aP < 0.05 and bP < 0.01 vs. Sham group, and cP < 0.05 and dP < 0.01 compared vs. Ovx group (one-way ANCOVA with Tukey’s honest significant difference test). BC, baseline control; SSI, Sesamum indicum; ANCOVA, analysis of covariance.

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