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Clinical and Experimental Obstetrics & Gynecology  2020, Vol. 47 Issue (1): 47-52    DOI: 10.31083/j.ceog.2020.01.4987
Original Research Previous articles | Next articles
Effect of the methanol extract of the Lion’s Mane mushroom, Hericium erinaceus, on bone metabolism in ovariectomized rats
N. Morita1, H. Matsushita1, *(), A. Minami2, S. Shimizu1, R. Tachibana1, H. Kanazawa3, T. Suzuki2, K. Watanabe1, A. Wakatsuki1
1Department of Obstetrics and Gynecology, School of Medicine, Aichi Medical University, Nagakute, Japan
2Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
3Department of Functional Anatomy, School of Nursing, University of Shizuoka, Shizuoka, Japan
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Abstract  

Purpose: Osteoporosis is a major health concern in postmenopausal women. The aim of the study was to investigate the effect of Hericium erinaceus (H. erinaceus), also known as the Lion’s Mane mushroom, consumption on bone metabolism in ovariectomized (Ovx) rats. Materials and Methods: Rats in the Baseline group were sacrificed immediately, whereas rats in the Sham group underwent sham surgery, and those in the Ovx and Ovx + HE groups underwent bilateral Ovx. A diet containing the methanol extract of H. erinaceus was given to the Ovx+HE group for 13 weeks. Results: There was no significant difference in the femoral bone mass between the Ovx and Ovx+HE groups. In the histomorphometric study of the proximal tibial metaphysis, the osteoblast surface, mineralizing surface, and fat cell number were lower in the Ovx+HE group than those in the Ovx group. Conclusion: The present findings suggest that H. erinaceus does not prevent but rather accelerates bone loss in Ovx rats.
Key words:  Adipocyte      Bone histomorphometry      Bone mineral density      Menopause      Mushroom      Rat     
Published:  15 February 2020     
*Corresponding Author(s):  H. Matsushita     E-mail:  hirosm@aichi-med-u.ac.jp
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N. Morita   
H. Matsushita   
A. Minami   
S. Shimizu   
R. Tachibana   
H. Kanazawa   
T. Suzuki   
K. Watanabe   
A. Wakatsuki   

Cite this article: 

N. Morita, H. Matsushita, A. Minami, S. Shimizu, R. Tachibana, H. Kanazawa, T. Suzuki, K. Watanabe, A. Wakatsuki. Effect of the methanol extract of the Lion’s Mane mushroom, Hericium erinaceus, on bone metabolism in ovariectomized rats. Clinical and Experimental Obstetrics & Gynecology, 2020, 47(1): 47-52.

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https://ceog.imrpress.com/EN/10.31083/j.ceog.2020.01.4987     OR     https://ceog.imrpress.com/EN/Y2020/V47/I1/47

Figure 1.  — (A) Percent fat volume (Fa.V/Ma.V), (B) fat cell number (N.Fa/Ma.V, /mm2), and (C) unit fat volume (Fa.V/N.Fa, µm2) of the right femur measured at the proximal tibial metaphysis in female Wistar rats at the beginning of the study (Baseline) and in shamoperated (Sham) or ovariectomized rats fed the H. erinaceus diet (Ovx+HE) or control diet (Ovx). Data are expressed as the means ± standard error of the mean. aP < 0.01 compared with the Sham group, and bP < 0.01 compared with the Ovx group (one-way analysis of covariance with Tukey’s honest significant difference test).

Table 1  — Bone mineral content and bone mineral density of the whole femur as well as the proximal, middle, and distal third of the femur in female Wistar rats at the beginning of the study (Baseline) and in sham-operated (Sham) or ovariec-tomized rats fed the H. erinaceus diet (Ovx+HE) or control diet (Ovx).
Baseline (n = 6) Sham (n = 10) Ovx (n = 10) Ovx+HE (n = 10)
BMC, g
Whole 0.225 ± 0.004 0.284 ± 0.005 0.281 ± 0.008b 0.280 ± 0.004a
Proximal third 0.083 ± 0.002 0.108 ± 0.002 0.103 ± 0.003a 0.104 ± 0.002a
Middle third 0.050 ± 0.000 0.065 ± 0.002 0.070 ± 0.002 0.070 ± 0.000

BMD, g/cm2		 Distal third 0.093 ± 0.002 0.113 ± 0.003 0.110 ± 0.003a 0.111 ± 0.002a
Whole 0.181 ± 0.002 0.202 ± 0.003 0.190 ± 0.002b 0.189 ± 0.002b
Proximal third 0.182 ± 0.002 0.206 ± 0.003 0.187 ± 0.002b 0.190 ± 0.001b
Middle third 0.149 ± 0.002 0.171 ± 0.002 0.171 ± 0.001 0.169 ± 0.002
Distal third 0.204 ± 0.002 0.219 ± 0.003 0.206 ± 0.003b 0.200 ± 0.004b
Table 2  — Structural and dynamic cancellous bone indices measured at the proximal tibial metaphysis in female Wistar rats at the beginning of the study (Baseline) and in sham-operated (Sham) or ovariectomized rats fed the H. erinaceus diet (Ovx+HE) or control diet (Ovx).
Baseline (n = 6) Sham (n = 10) Ovx (n = 10) Ovx+HE (n = 10)
Structural indices
Bone volume, %
21.7 ± 0.9
29.7 ± 1.7
8.0 ± 0.8b
10.3 ± 0. 8b
Trabecular thickness, µm 58.6 ± 1.8 69.7 ± 3.0 61.8 ± 1.4a 65.9 ± 2.9
Trabecular number, /mm 3.69 ± 0.09 4.25 ± 0.12 1.29 ± 0.10b 1.57 ± 0.11b
Osteoid surface, % 23.1 ± 2.1 18.5 ± 2.1 31.8 ± 2.3b 27.6 ± 2.0b
Osteoid volume, % 3.00 ± 0.34 2.11 ± 0.33 4.28 ± 0.49b 3.48 ± 0.46a
Osteoblast surface, % 3.47 ± 0.44 1.13 ± 0.25 3.15 ± 0.65b 1.97 ± 0.49a, d
Eroded surface, % 7.51 ± 1.02 3.32 ± 0.16 4.04 ± 0.35 4.17 ± 0.66
Osteoclast surface, % 4.64 ± 0.69 0.65 ± 0.11 1.29 ± 0.12 1.64 ± 0.48
Dynamic indices
Single-labeled surface, %
37.4 ± 3.5
25.2 ± 1.5
25.7 ± 0.9
22.3 ± 0.9
Double-labeled surface, % 19.3 ± 3.0 13.3 ± 1.3 21.6 ± 1.0b 19.0 ± 1.2a
Mineralizing surface, % 38.0 ± 2.1 26.0 ± 1.4 34.4 ± 1.2b 30.1 ± 1.3d
Mineral apposition rate, µm/day 2.05 ± 0.18 1.37 ± 0.03 1.40 ± 0.05 1.41 ± 0.05
Bone formation rate, mm3/mm2/year 0.290 ± 0.042 0.131 ± 0.009 0.177 ± 0.010 0.156 ± 0.009
Bone formation rate, %/year 1013 ± 153 382 ± 30 579 ± 44a 480 ± 37
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