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Clinical and Experimental Obstetrics & Gynecology  2017, Vol. 44 Issue (4): 535-539    DOI: 10.12891/ceog3426.2017
Original Research Previous articles | Next articles
The effects of sildenafil and tadalafil on ischemia–reperfusion injury in rat ovarian torsion model
C. Togrul1, *(), A.C. Dogan2, E. Baser2, M. Dogan3, A. Albayrak4, M. Caglar5, T. Gungor1, G. Ozturk6, 8, E. Fadillioglu3, T. Delibasi7, 8, 9
1 Department of Obstetrics and Gynecology, Medical Faculty of Hitit University, Corum, Turkey
2 Department of Obstetrics and Gynecology, Zekai Tahir Burak Women’s Health Training and Research Hospital, Ankara, Turkey
3 Department of Physiology, Medical Faculty of Hacettepe University, Ankara, Turkey
4 Department of Pathology, Diskapi YB Training and Research Hospital, Ankara, Turkey
5 Department of Obstetrics and Gynecology, Medical Faculty of Duzce University, Duzce, Turkey
6 Department of Biochemistry, Diskapi YB Training and Research Hospital, Ankara, Turkey
7 Department of Endocrinology, Diskapi YB Training and Research Hospital, Ankara, Turkey
Pancreas Islet Research Center, Diskapi YB Training and Research Hospital, Ankara, Turkey
9 Department of Endocrinology and Metabolism, Kastamonu Medical Faculty of Hacettepe University, Ankara, Turkey
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Abstract  Objective: In cases of ovarian torsion (OT), reperfusion injury may further damage ovarian tissue. Tadalafil and sildenafil are phosphodiesterase-5 (PDE-5) inhibitors that are primarily used for treatment of erectile dysfunction and pulmonary hypertension. In this study, the authors investigated the effects of these agents in preventing ovarian ischemia-reperfusion injury in a rat OT model. Materials and Methods: A total of 48 young adult female Wistar Albino rats were randomized into six groups (n=8 in each group); group 1: controlsham operated, group 2: sildenafil one mg/kg, group 3: tadalafil one mg/kg, group 4: sildenafil one mg/kg + ischemia/reperfusion, (I/R) two hours of ischemia and two hours of reperfusion and a single dose of oral one mg/kg sildenafil one hour before I/R, group 5: I/R, and group 6: tadalafil one mg/kg + I/R two hours of ischemia and two hours of reperfusion and a single dose of oral one mg/kg tadalafil one hour before I/R. Histopathologic evaluation was performed and scored according to the degree of congestion, edema, and hemorrhage. Total histological score (THS), tissue protein carbonyl (PC), malondialdehyde (MDA) levels, and catalase (CAT) activity were also calculated. Results: Group 4, group 5, and group 6 had similar congestion, edema and THS (p > 0.05). Group 6 had significantly lower hemorrhage score, when compared with group 5 (p = 0.03). Mean PC, MDA levels, and CAT were similar between groups 4, 5, and 6 (p > 0.05). Conclusions: Tadalafil was associated with a decreased tissue hemorrhage score in I/R injury in rat ovarian torsion model.
Key words:  Reperfusion injury      Phosphodiesterase inhibitors      Ovary     
Published:  10 August 2017     
*Corresponding Author(s):  C.TOGRUL     E-mail:  cihantugrul@yahoo.com

Cite this article: 

C. Togrul, A.C. Dogan, E. Baser, M. Dogan, A. Albayrak, M. Caglar, T. Gungor, G. Ozturk, E. Fadillioglu, T. Delibasi. The effects of sildenafil and tadalafil on ischemia–reperfusion injury in rat ovarian torsion model. Clinical and Experimental Obstetrics & Gynecology, 2017, 44(4): 535-539.

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https://ceog.imrpress.com/EN/10.12891/ceog3426.2017     OR     https://ceog.imrpress.com/EN/Y2017/V44/I4/535

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