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Clinical and Experimental Obstetrics & Gynecology  2014, Vol. 41 Issue (6): 620-626    DOI: 10.12891/ceog17492014
Original Research Previous articles | Next articles
Proteomic investigation of the severe preeclampsia treatment by low molecular weight heparin
Shuoshi Wang1, 2, Shuiwang Hu3, Mei Zhong2, *()
1The Department of Obstetrics and Gynecology, 2nd Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen
2The Department of Obstetrics and Gynecology, Southern Medical University, Nanfang Hospital, Guangzhou
3Department of Pathophysiology, Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou (China)
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Abstract  
Purpose: The primary goal of this study is to investigate the mechanism of severe preeclampsia (PE) treatment by low molecular weight heparin (LMWH). Materials and Methods: Using two-dimensional difference in-gel electrophoresis (2D-DIGE) combined with matrix assisted laser desorption ionization-time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF) approach to identify the proteins that expressed differently in the serum samples of five patients before and after subcutaneous injection of LMWH (0.4 ml/person). Results: Seven protein spots were identified in 2D-DIGE that show significant change in expression level after LMWH treatment. Further analysis of seven protein spots with MALDI-TOF/TOF identified six different proteins. To confirm the proteomic data, two meaningful proteins of the six proteins, alpha-1-acid glycoprotein (AGP) and serotransferrin are subjected to immunoblotting. All of the proteins are obviously down-regulated after LMWH treatment. Conclusions: PE is a pregnancy-specific disease that clinically manifests as new-onset hypertension and proteinuria after 20 weeks of gestation. LMWH is an effective treatment of severe PE. The present proteomics based investigation may provide a new angle to understand the mechanism of severe PE treatment with LMWH.
Key words:  Severe preeclampsia      LMWH      DIGE      MALDI-TOF/TOF      Human serum     
Published:  10 December 2014     
*Corresponding Author(s):  MEI ZHONG     E-mail:  doctorzhongmei@gmail.com

Cite this article: 

Shuoshi Wang, Shuiwang Hu, Mei Zhong. Proteomic investigation of the severe preeclampsia treatment by low molecular weight heparin. Clinical and Experimental Obstetrics & Gynecology, 2014, 41(6): 620-626.

URL: 

https://ceog.imrpress.com/EN/10.12891/ceog17492014     OR     https://ceog.imrpress.com/EN/Y2014/V41/I6/620

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